Nutrición Hospitalaria 02600 / http://dx.doi.org/10.20960/nh.02600
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Effect on nutritional markers of a model of aberrant crypt foci induced by azoxymethane and sodium dextran sulfate in Sprague Dawley rats


Aarón Kuri-García, Alejandra González-Reyes, Paola Jazmín Aranda-Vargas, Ulisses Moreno Celis, Carmen Mejía, Teresa García-Gasca, Roberto Augusto Ferríz-Martínez, Karina de la Torre-Carbot, Carlos Saldaña Gutiérrez, Jorge Luis Chávez-Servín

Prepublicado: 2019-09-11
Publicado: 2019-10-15

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Introduction: aberrant crypt foci (ACF) are colon preneoplastic lesions that can be used as a tool to study preventive processes for colorectal cancer (CRC). This model consists of initiation induced by azoxymethane (AOM) and promoted by sodium dextran sulfate (DSS), simulating human colonic carcinogenesis in a rat model. There is no direct information on the effects of this process on nutritional markers. Objective: to determine the effect on nutritional markers after the induction of ACF by AOM/DSS in a rat model. Methods: ACF were induced in 24 four-week-old Sprague Dawley male rats by administration of 2 AOM injections (10 mg/kg) and 7 days of 2% DSS in their drinking water. Body weight gain, food and fluid intake, weight of sacrificial organs, nutritional biochemical profiles, liver and kidney toxicity were evaluated. Cell counts in blood were also performed and histological sections evaluated in specific organs. The model was confirmed with identification and counts of ACF. Half of the rats were sacrificed at the sub-chronic stage and the rest at the chronic stage. Results: at the sub-chronic stage, changes in the liver and colon weight, and in the lymphocyte count were observed. For both stages, histopathological damage was observed in liver, kidney and colon, along with alterations in serum glucose levels. Conclusions: the model for proposed ACF can be used at the sub-chronic stage without the need for observation at the chronic stage. More research is needed to determine the mechanism of the observed effects.

Palabras Clave: Azoxymethane. Dextran sulfate sodium. Nutritional effects.



Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018 Nov 1;68(6):394–424.
Papanikolaou A, Wang QS, Papanikolaou D, Whiteley HE, Rosenberg DW. Sequential and morphological analyses of aberrant crypt foci formation in mice of differing susceptibility to azoxymethane-induced colon carcinogenesis. Carcinogenesis. 2000 Aug;21(8):1567–72.
Tanaka T. Colorectal carcinogenesis: Review of human and experimental animal studies. J Carcinog. 2009;8:5.
Thaker AI, Shaker A, Rao MS, Ciorba MA. Modeling Colitis-Associated Cancer with Azoxymethane (AOM) and Dextran Sulfate Sodium (DSS). J Vis Exp. 2012 Sep 11;(67).
Martínez Gómez MJ, Melián Fernández C, Romeo Donlo M. Nutrición en enfermedad inflamatoria intestinal. Nutr Hosp. 2016 Jul 12;33(4):59–62.
Brasil Lopes M, Rocha R, Castro Lyra A, Rosa Oliveira V, Gomes Coqueiro F, Silveira Almeida N, et al. Restriction of dairy products; a reality in inflammatory bowel disease patients. Nutr Hosp. 2014 Mar 1;29(3):575–81.
Mori H, Yamada Y, Hirose Y, Kuno T, Katayama M, Sakata K, et al. Chemoprevention of large bowel carcinogenesis; the role of control of cell proliferation and significance of beta-catenin-accumulated crypts as a new biomarker. Eur J Cancer Prev. 2002 Aug;11 Suppl 2:S71-5.
De Robertis M, Massi E, Poeta ML, Carotti S, Morini S, Cecchetelli L, et al. The AOM/DSS murine model for the study of colon carcinogenesis: From pathways to diagnosis and therapy studies. J Carcinog. 2011 Mar 24;10(1):9.
Kobaek-Larsen M, Fenger C, Ritskes-Hoitinga J. Secondary effects induced by the colon carcinogen azoxymethane in BDIX rats. APMIS. 2004 Jun 1;112(6):319–29.
Periasamy S, Liu C-T, Wu W-H, Chien S-P, Liu M-Y. Dietary Ziziphus jujuba Fruit Influence on Aberrant Crypt Formation and Blood Cells in Colitis-Associated Colorectal Cancer in Mice. Asian Pac J Cancer Prev. 2015;16(17):7561–6.
Hoensch HP, Kirch W. Potential Role of Flavonoids in the Prevention of Intestinal Neoplasia: A Review of Their Mode of Action and Their Clinical Perspectives. Int J Gastrointest Cancer. 2005;35(3):187–96.
Pan M-H, Lai C-S, Wu J-C, Ho C-T. Molecular mechanisms for chemoprevention of colorectal cancer by natural dietary compounds. Mol Nutr Food Res. 2011 Jan 1;55(1):32–45.
Celada P, Bastida S, Sánchez-Muniz FJ. To eat or not to eat meat. That is the question. Nutr Hosp. 2016 Jan 1;33(1):177–81.
Animals NRC (US) C for the U of the G for the C and U of L. Guide for the Care and Use of Laboratory Animals. Guide for the Care and Use of Laboratory Animals. National Academies Press (US); 2011.
Bird RP, Good CK. The significance of aberrant crypt foci in understanding the pathogenesis of colon cancer. Toxicol Lett. 2000 Mar 15;112–113:395–402.
Feregrino-Perez AA, Piñol-Felis C, Gomez-Arbones X, Guevara-González RG, Campos-Vega R, Acosta-Gallegos J, et al. A Non-digestible Fraction of the Common Bean (Phaseolus vulgaris L.) Induces Cell Cycle Arrest and Apoptosis During Early Carcinogenesis. Plant Foods Hum Nutr. 2014 Sep 21;69(3):248–54.
Boateng JA, Verghese M, Walker LT, Shackelford LA, Chawan CB. Inhibitory effects of selected dry beans (Phaseolus spp L) on azoxymethane-induced formation of aberrant crypt foci in Fisher 344 male rats. Nutr Res. 2007 Oct 1;27(10):640–6.
Moreno-Celis U, López-Martínez J, Blanco-Labra A, Cervantes-Jiménez R, Estrada-Martínez LE, García-Pascalin AE, et al. Phaseolus acutifolius Lectin Fractions Exhibit Apoptotic Effects on Colon Cancer: Preclinical Studies Using Dimethilhydrazine or Azoxi-Methane as Cancer Induction Agents. Molecules. 2017 Oct 8;22(10):1670.
Magnuson BA, Carr I, Bird RP. Ability of aberrant crypt foci characteristics to predict colonic tumor incidence in rats fed cholic acid. Cancer Res. 1993 Oct 1;53(19):4499–504.
Corrales A, Jaramillo G, Rodrguez B, Yahia E, Maldonado M. Mango (Mangifera indica cv. Azucar) antiinflammatory and chemopreventive role during colorectal carcinogenesis. Emirates J Food Agric. 2016;28(10):704.
Caderni G, Bollito E, Elashoff RM, Tessitore L. Colon Cancer Is Induced by a Single Low Dose of Azoxymethane in Fasted-Refed Rats. Nutr Cancer. 1999 Nov;35(2):137–42.
Villa A, Connell CL, Abati S. Diagnosis and management of xerostomia and hyposalivation. Ther Clin Risk Manag. 2015;11:45–51.
Burlamaqui IMB, Dornelas CA, Almeida PRC, Jamacaru FVF, Mota DMC, Mesquita FJC, et al. Repercussão hepática da carcinogênese colorretal induzida pelo azoximetano. Rev Col Bras Cir. 2013 Apr;40(2):137–41.
Perše M, Cerar A. Morphological and molecular alterations in 1,2 dimethylhydrazine and azoxymethane induced colon carcinogenesis in rats. J Biomed Biotechnol. 2011;2011:473964.
Kim J-H, Hue J-J, Kang BS, Park H, Nam SY, Yun YW, et al. Effects of selenium on colon carcinogenesis induced by azoxymethane and dextran sodium sulfate in mouse model with high-iron diet. Lab Anim Res. 2011 Mar;27(1):9–18.
Matkowskyj KA, Marrero JA, Carroll RE, Danilkovich A V., Green RM, Benya R V. Azoxymethane-induced fulminant hepatic failure in C57BL/6J mice: characterization of a new animal model. Am J Physiol Liver Physiol. 1999 Aug;277(2):G455–62.
Hata K, Kubota M, Shimizu M, Moriwaki H, Kuno T, Tanaka T, et al. Monosodium glutamate-induced diabetic mice are susceptible to azoxymethane-induced colon tumorigenesis. Carcinogenesis. 2012 Mar 1;33(3):702–7.
Hirose Y, Hata K, Kuno T, Yoshida K, Sakata K, Yamada Y, et al. Enhancement of development of azoxymethane-induced colonic premalignant lesions in C57BL/KsJ-db/db mice. Carcinogenesis. 2003 Dec 19;25(5):821–5.
Sunter JP, Senior P V. Induction of renal tumours in rats by the administration of 1,2 dimethylhydrazine. J Pathol. 1983 May 1;140(1):69–76.

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