Nutrición Hospitalaria 00643 / http://dx.doi.org/10.20960/nh.643

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Effect of glutamine on liver injuries induced by intestinal ischemia-reperfusion in rats

Renata Hartmann, Francielli Licks, Elizângela Gonçalves Schemitt, Josieli Raskopf Colares, Juliana Da Silva, Rosa Maria Moura, Gilmara Pandolfo Zabot, Henrique Sarubbi Fillmann, Norma Possa Marroni

VOLUMEN 34, NÚM. 3, mayo-junio (2017), PAG. 548-554

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Introduction: Intestinal ischemia-reperfusion (I/R) injury may cause cell and tissue damage, reaching also other organs such as the liver. Because of the involvement of free radicals in I/R injury, treatment options with antioxidants have been studied and tested.Objective: To evaluate the effect of glutamine (Gln) in the liver of animals with intestinal I/R injury.Methods: We used 20 male Wistar rats divided into four groups: sham-operated (SO); glutamine + sham-operated (G+SO); intestinal ischemia-reperfusion (I/R); glutamine + intestinal ischemia-reperfusion (G+I/R). The superior mesenteric artery was clamped for 30 minutes and reperfused for 15 minutes. Gln (25 mg/kg/day) diluted in 1 ml of saline was administered intraperitoneally on the two days before I/R induction.Results: Levels of serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), and alkaline phosphatase (ALP), lipid peroxidation (LPO) and expressions of interleukin-6 (IL-6) and nuclear factor kappa B (NF-kB) showed a significant reduction in the G+I/R group as compared with the I/R group. The activity of catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), and the levels of glutathione (GSH) showed an increase in the G+I/R group as compared with the I/R group.Conclusion: Pretreatment with Gln reduced oxidative, tissue damage and showed a decrease expression of inflammatory mediators.

Palabras Clave: DNA damage. Inflammation. Lipid peroxidation. Oxidative stress.

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Trabajo Original

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