Nutrición Hospitalaria 03432 /
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Time-restricted feeding in dark phase of circadian cycle and/or westernized diet cause mixed hyperlipidemia in rats

Eryka Maria dos Santos Alves, Nathalia Caroline de Oliveira Melo, Ana Carolina Oliveira Costa, Humberto de Moura Barbosa, Nathália Cavalcanti de Morais Araújo, Gisélia de Santana Muniz, Elizabeth do Nascimento

Prepublicado: 2021-01-22
Publicado: 2021-04-12

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Background: the dietary pattern that characterizes western diet is strongly associated with metabolic diseases and excess weight, as well as chronic illnesses. Misaligned feeding schedules can lead to or aggravate the development of such conditions. Aim: this study evaluated the influence of dietary composition and/or time-restricted feeding on the anthropometric and biochemical profile of adult rats. Methods: forty male rats, at 60 days of life, were divided into the following groups: Control (C), Restricted Control (RC), Westernized (W), and Restricted Westernized (RW). Results: westernized groups, in spite of a low energy intake (C = 5399 ± 401.2 kcal; RC = 4279.0 ± 476.2 kcal; W = 4302 ± 619.8 kcal; RW = 4081.0 ± 404.4 kcal, p < 0.001), had a higher body weight (C = 404.6 ± 39.1 g; RC = 335.1 ± 36.5 g; W = 488.9 ± 51.2 g; RW = 438.8 ± 36.5 g, p < 0.001) as compared to their paired controls (RC and C) — around 30 % and 20 % more for RW and W, respectively. The westernized diet caused glucose intolerance and mixed hyperlipidemia, characterized by higher concentrations of cholesterol (C = 40.8 ± 7.4 mg/dL; RC = 76.7 ± 10.8 mg/dL; W = 61.3 ± 20.2 mg/dL; RW = 42.2 ± 8.2 mg/dL), LDLc (C = 17.4 ± 7.5 mg/dL; RC = 38.8 ± 7.2 mg/dL ; W = 45.3 ± 15.8 mg/dL; RW = 11.0 ± 5.8 mg/dL), and triacylglycerol (C = 45.2 ± 15.0 mg/dL; RC = 73.2 ± 21.5 mg/dL ; W = 83.6 ± 23.4 mg/dL; RW = 57.5 ± 13.6 mg/dL) in the serum (p < 0.05). Conclusion: the effect of time-restricted feeding on body weight was strongly dependent on diet composition. The glucose tolerance test showed an influence of the circadian cycle phase. Mixed hyperlipidemia varied according to the presence of westernized diet and/or time-restricted food.

Palabras Clave: Hyperlipidemia. Time-restricted feeding. Westernized diet. Adult rats.

Nazni P. Association of western diet & lifestyle with decreased fertility. Indian J Med Res [Internet] 2014;140:S78-81. Available at:
Nascimento CMO, Ribeiro EB, Oyama LM. Metabolism and secretory function of white adipose tissue: effect of dietary fat. An Acad Bras Cienc 2009;81:453-66.
Ferro Cavalcante TC, Marcelino Da Silva AA, Alheiros Lira MC, Do Amaral Almeida LC, Jaques Marques AP, Do Nascimento E. Early exposure of dams to a westernized diet has long-term consequences on food intake and physiometabolic homeostasis of the rat offspring. Int J Food Sci Nutr 2014;65(8):989-93.
Brasil. Ministério da Saúde. Secretaria de Vigilância em Saúde. Departamento de Vigilância de Doenças e Agravos não Transmissíveis e Promoção da Saúde. Vigitel Brasil 2017: vigilância de fatores de risco e proteção para doenças crônicas por inquérito telefônico: Brasília: Ministério da Saúde; 2018.
Czech MP. Insulin action and resistance in obesity and type 2 diabetes. Nat Med 2017;23(7):804-14.
Silva JM, Saldanha C. Dieta, aterosclerose e complicações aterotrombóticas. Rev Port Cardiol 2007;26(3):277-94.
Tain YL, Sheen JM, Yu HR, Chen CC, Tiao MM, Hsu CN, et al. Maternal melatonin therapy rescues prenatal dexamethasone and postnatal high-fat diet induced programmed hypertension in male rat offspring. Front Physiol 2015;6(12):1-8.
Baron KG, Reid KJ, Kern AS, Zee PC. Role of sleep timing in caloric intake and BMI. Obesity 2011;19(7):1374-81.
US Departament of Agriculture. US Department of Agriculture ARS, Beltsville Human Nutrition Research Center, Food Surveys Research Group & US Department of Health and Human Services, Centers for Disease Control and Prevention, National Center for Health Statistics. What We Eat in America 2012. Available at:
Rocha LS da, de Matos RJB, de Souza JA, Costa ACO, Lopes de Souza S, Muniz G de S, et al. Daytime increase in caloric intake without change in total 24-h caloric intake can increase adiposity but not total bodyweight in rats with inverted feeding pattern. Appl Physiol Nutr Metab 2017;42(9):931-40.
Yoshino J, Klein S. A Novel Link Between Circadian Clocks and Adipose Tissue Energy Metabolism. Am Diabetes Assoc 2013;62(7):2175-7.
Tahara Y, Shibata S. Chronobiology and nutrition [Internet]. Vol. 253, Neuroscience. IBRO; 2013. p. 78–88. Available at:
Poggiogalle E, Jamshed H, Peterson CM. Circadian regulation of glucose, lipid, and energy metabolism in humans. Metabolism [Internet] 2018;84:11-27. Available at:
Bayne K. Revised Guide for the Care and Use of Laboratory Animals available. Vol. 39, American Physiological Society Physiologist Physiologist; 1996. p. 208-211.
Reeves PG, Suppl M. Symposium : Animal Diets for Nutritional and Toxicological Research Components of the AIN-93 Diets as Improvements in the AIN-76A Diet 1 , 2. Exp Biol 1997;127:838-41.
Chaix A, Zarrinpar A, Miu P, Panda S. Time-restricted feeding is a preventative and therapeutic intervention against diverse nutritional challenges. Cell Metab 2014;20(6):991-1005.
Ferro Cavalcante TC, Lima Da Silva JM, Da Marcelino Da Silva AA, Muniz GS, Da Luz Neto LM, Lopes De Souza S, et al. Effects of a westernized diet on the reflexes and physical maturation of male rat offspring during the perinatal period. Lipids 2013;48(11):1157-68.
Novelli ELB, Diniz YS, Galhardi CM, Ebaid GMX, Rodrigues HG, Mani F, et al. Anthropometrical parameters and markers of obesity in rats. Lab Anim 2007;41(1):111-9.
Andrikopoulos S, Blair AR, Deluca N, Fam BC, Proietto J. Evaluating the glucose tolerance test in mice. Am J Physiol-Endocrinol Metab 2008;295(6):1323-32.
Le Floch JP, Escuyer P, Baudin E, Baudon D, Perlemuter L. Blood glucose area under the curve. Methodological aspects. Diabetes Care 1990;13(2):172-5.
Nascimento E, Guzman-Quevedo O, Delacourt N, da Silva Aragão R, Perez-Garcia G, de Souza SL, et al. Long-Lasting Effect of Perinatal Exposure to L-tryptophan on Circadian Clock of Primary Cell Lines Established from Male Offspring Born from Mothers Fed on Dietary Protein Restriction. PLoS One 2013;8(2).
William T. Friedewald, Levy RI, Fredrickson DS. Estimation of the Concentration of Low-Density Lipoprotein Cholesterolin Plasma, Without Use of the Preparative Ultracentrifuge. Clin Chem 1972;18(6):499-502.
Buettner R, Scholmerich J, Bollheimer LC. High-fat Diets: Modeling the Metabolic Disorders of Human Obesity in Rodents. Obesity 2007;15(4):798-808.
MacLean PS, Higgins JA, Johnson GC, Fleming-Elder BK, Donahoo WT, Melanson EL, et al. Enhanced metabolic efficiency contributes to weight regain after weight loss in obesity-prone rats. Am J Physiol - Regul Integr Comp Physiol 2004;287(6 56-6).
Schilperoort M, van den Berg R, Dollé MET, van Oostrom CTM, Wagner K, Tambyrajah LL, et al. Time-restricted feeding improves adaptation to chronically alternating light-dark cycles. Sci Rep 2019;9(1):1-12.
Angéloco LRN, Deminice R, Leme IDA, Lataro RC, Jordão AA. Bioelectrical impedance analysis and anthropometry for the determination of body composition in rats: Effects of high-fat and high-sucrose diets. Rev Nutr 2012;25(3):331-9.
Little TJ, Horowitz M, Feinle-Bisset C. Modulation by high-fat diets of gastrointestinal function and hormones associated with the regulation of energy intake: Implications for the pathophysiology of obesity. Am J Clin Nutr 2007;86(3):531-41.
Araújo CRR, Esteves EA, Dessimoni-Pinto NAV, Batista ÂG. Myrciaria cauliflora peel flour had a hypolipidemic effect in rats fed a moderately high-fat diet. J Med Food 2014;17(2):262-7.
Gault VA, Bhat VK, Irwin N, Flatt PR. A novel glucagon-like peptide-1 (GLP-1)/Glucagon hybrid peptide with triple-acting agonist activity at glucose-dependent insulinotropic polypeptide, GLP-1, and glucagon receptors and therapeutic potential in high fat-fed Mice. J Biol Chem 2013;288(49):35581-91.
Lane MD, Wolfgang M, Cha SH, Dai Y. Regulation of food intake and energy expenditure by hypothalamic malonyl-CoA. Int J Obes 2008;32:S49-54.
Gao S, Kinzig KP, Aja S, Scott KA, Keung W, Kelly S, et al. Leptin activates hypothalamic acetyl-CoA carboxylase to inhibit food intake. Proc Natl Acad Sci 2007;104(44):17358-63.
Wolfgang MJ, Cha SH, Sidhaye A, Chohnan S, Cline G, Shulman GI, et al. Regulation of hypothalamic malonyl-CoA by central glucose and leptin. Proc Natl Acad Sci 2007;104(49):19285-90.
Lopez M, Lelliott CJ, Tovar S, Kimber W, Gallego R, Virtue S, et al. Tamoxifen-Induced Anorexia Is Associated With Fatty Acid Synthase Inhibition in the Ventromedial Nucleus of the Hypothalamus and Accumulation of Malonyl-CoA. Diabetes 2006;55(5):1327-36.
La Fleur SE, Kalsbeek A, Wortel J, Fekkes ML, Buijs RM. A daily rhythm in glucose tolerance: A role for the suprachiasmatic nucleus. Diabetes 2001;50(6):1237-43.
Stenvers DJ, Scheer FAJL, Schrauwen P, la Fleur SE, Kalsbeek A. Circadian clocks and insulin resistance. Nat Rev Endocrinol 2019;15(2):75-89.
Rudic RD, McNamara P, Curtis AM, Boston RC, Panda S, Hogenesch JB, et al. BMAL1 and CLOCK, two essential components of the circadian clock, are involved in glucose homeostasis. PLoS Biol 2004;2(11).
Hatori M, Vollmers C, Zarrinpar A, DiTacchio L, Bushong EA, Gill S, et al. Time-restricted feeding without reducing caloric intake prevents metabolic diseases in mice fed a high-fat diet. Cell Metab 2012;15(6):848-60.
Puri V, Ranjit S, Konda S, Nicoloro SMC, Straubhaar J, Chawla A, et al. Cidea is associated with lipid droplets and insulin sensitivity in humans. Proc Natl Acad Sci USA 2008;105(22):7833-8.
Zhang W, Patil S, Chauhan B, Guo S, Powell DR, Le J, et al. FoxO1 regulates multiple metabolic pathways in the liver effects on gluconeogenic, glycolytic, and lipogenic gene expression. J Biol Chem 2006;281(15):10105-17.
Kimmel AR, Sztalryd C. The Perilipins: Major Cytosolic Lipid Droplet–Associated Proteins and Their Roles in Cellular Lipid Storage, Mobilization, and Systemic Homeostasis. Annu Rev Nutr 2016;36(1):471-509.

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