Nutrición Hospitalaria 00039 / http://dx.doi.org/10.20960/nh.39

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Trabajo Original

Protection by polyphenol extract from olive stones against apoptosis produced by oxidative stress in human neuroblastoma cells

Ernesto Cortés-Castell, Carmen Veciana-galindo, Luis Torró-montell, Antonio Palazón-Bru, Elia Sirvent-Segura, Vicente Gil-guillén, Mercedes Rizo-baeza

VOLUMEN 33, NÚM. 1, enero-febrero (2016), PAG. 123-130

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Objective: We evaluated the protective activity of an extract from a by-product such as olive stones, through its ability to inhibit H202 induced apoptosis in the SH-SY5Y human neuroblastoma cell line.Material and methods: To such end, 20,000 cells/well were cultivated and differentiation with retinoic acid was initiated. Once the cells were differentiated, apoptosis was induced with and without H2O2 extract. Finally, cDNA extraction was performed, and pro-apoptotic genes Bax and anti-apoptotic genes Bcl-2 were analyzed. Quantification of the gene expression was performed using the GAPDH gene marker.Results: Cell viability with the extract is 97.6% (SD 5.7) with 10 mg/l and 62.8% (SD 1.2) to 50 mg/l, using 10 mg/l for the biomarker assay. The retinoic acid differentiated SH-S cell line (10 μM) shows a clear apoptosis when treated with H2O2 150 μM, with a Bax/Bcl-2 ratio of 3.75 (SD 0.80) in contrast to the differentiated control cells subjected to H2O2 and with extract, which have the same ratio of 1.02 (SD 0.01-0.03).Conclusion: The olive stone extract shows anti-apoptotic activity in the provoked cell death of SH-SY5Y human neuroblastoma cells in their normal state, defending them from oxidative stress which produces a significant increase in the apoptotic gene ratio in contrast to anti-apoptotic genes (Bax/Bcl-2).

Palabras Clave: Polyphenols; Olive stones; Apoptosis; Human neuroblastome cells

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El Fessikh, Meriem, Elhrech, Hamza, El Yahyaoui El Idrissi, Amina, Lee, Learn-Han, Abdulmonem, Waleed Al, El Omari, Nasreddine, Bouyahya, Abdelhakim (2025) Sustainable valorization of olive stone by-products: Opportunities and challenges. Journal of Food Composition and Analysis

López Martínez, Aránzazu, Suárez González, Marta, Menéndez Llames, Begoña, Palomo Moraleda, Pilar, González Muñiz, Soledad, Díaz Martín, Juan José (2022) Cardiovascular risk and insulin resistance in childhood leukemia survivors. Nutrición Hospitalaria

Mumcu, Ayla, Deliboran, Aise (2025) Olive stone as a sustainable agricultural by-product: Valorization pathways and prospects in food and feed Industries. The North African Journal of Food and Nutrition Research

Gao, Chen, Zhou, Yinglu, Li, Huatao, Cong, Xia, Jiang, Zhongling, Wang, Xin, Cao, Rongfeng, Tian, Wenru (2017) Antitumor effects of baicalin on ovarian cancer cells through induction of cell apoptosis and inhibition of cell migration in vitro. Molecular Medicine Reports

Tangney CC, Rasmussen HE. Polyphenols, inflammation, and cardiovascular disease. Curr Atheroscler Rep 2013;15:324.
DOI: 10.1007/s11883-013-0324-x

Bartlett H, Eperjesi F. Age-related macular degeneration and nutritional supplementation: a review of randomised controlled trials. Ophthalmic Physiol Opt 2003;23:383-99.
DOI: 10.1046/j.1475-1313.2003.00130.x

Bleys J, Miller E, Pastor-Barriuso R, Appel L, Guallar E. Vitamin-mineral supplementation and the progression of atherosclerosis: a meta-analysis of randomized controlled trials. Am J Clin Nutr 2006;84:880-7.
DOI: 10.1093/ajcn/84.4.880

De Marco E, Savarese M, Paduano A, Sacchi R. Characterization and fractionation of phenolic compounds extracted from olive oil mill wastewaters. Food Chem 2007;104:858-67.
DOI: 10.1016/j.foodchem.2006.10.005

Pereira-Caro G, Madrona A, Mateos R, Rodríguez G, Trujillo M, et al. Synthesis of hydroxytyrosyl alkyl ethers from olive oil waste waters. Molecules 2009;14:1762-72.
DOI: 10.3390/molecules14051762

Halliwell B, Rafter J, Jenner A. Health promotion by flavonoids, tocopherols, tocotrienols, and other phenols: direct or indirect effects? Antioxidant or not? Am J Clin Nutr 2005;81:268S–276S.

Obied HK, Prenzler PD, Konczak I, Rehman AU, Robards K. Chemistry and bioactivity of olive biophenols in some antioxidant and antiproliferative in vitro bioassays. Chem Res Toxicol 2009;22:227-34.
DOI: 10.1021/tx8004168

De-la-Puerta R, Martinez-Dominguez ME, Ruiz-Gutierrez V. Effects of virgin olive oil phenolics on scavenging of reactive nitrogen species and upon nitergic neurotransmission. Life Sci 2001;69:1213-22.
DOI: 10.1016/S0024-3205(01)01218-8

Covas MI, Fito M, Lamuela-Raventos RM, Sebastia N, De-la-Torre-Boronat C, et al. Virgin olive oil poliphenolic compounds: binding to human low density lipoprotein (LDL) and effect on LDL oxidation. Int J Pharm Res 2000;20:49-54.

Owen RW, Haubner R, Wurtele G, Hull E, Spiegelhader B, et al. Olives and olive oil in cancer prevention. Eur J Cancer Prev 2004;13:319-26.
DOI: 10.1097/01.cej.0000130221.19480.7e

Quiles JL, Huertas JR, Ochoa JJ, Battino M, Mataix J, et al. Dietary fat (virgin olive oil or sunflower oil) and physical training interactions on blood lipids in the rat. Nutr 2003;19:363-8.
DOI: 10.1016/S0899-9007(02)00949-8

D’Angelo S, Ingrosso D, Migiarrdi V, Sorrentino A, Donnarumma G, et al. Hydroxytyrosol, a natural antioxidant from olive oil, prevents protein damage induced by long-wave ultraviolet radiation in melanoma cells. Free Radic Biol Med 2005;38:908-19.
DOI: 10.1016/j.freeradbiomed.2004.12.015

Petroni A, Blasevich M, Salami M, Papini N, Montedoro GF, et al. Inhibition of platelet aggregation and eicosanoid production by phenolic components of olive oil. Thromb Res 1995;78:151-60.
DOI: 10.1016/0049-3848(95)00043-7

Furneri PM, Piperno A, Sajia A, Bisignano G. Antimycoplasmas activity of hydroxytyrosol. Antimicrob Agents Chemother 2004;48:4892-4.
DOI: 10.1128/AAC.48.12.4892-4894.2004

Vauzour D, Corona G, Spencer JP. Caffeic acid, tyrosol and p-coumaric acid are potent inhibitors of 5-S-cysteinyl-dopamine induced neurotoxicity. Arch Biochem Biophys 2010;501:106-11.
DOI: 10.1016/j.abb.2010.03.016

Cortés E, Veciana C, Torro L, Sirvent E, Rizo MM et al. Actividad antiinflamatoria de un extracto polifenólico de hueso de olivas en la línea celular de monocitos humanos THP1-XBLUE-CD14. Nutr Hosp 2014;30:113-7.

Cortés E, Veciana C, Torro L, Sirvent E, Rizo MM, et al. Efecto sobre el neurodesarrollo y neuroprotección en pez cebra de un extracto polifenólico de huesos de aceituna. Nutr Hosp 2014;30:338-42.

Cheung YT, Lau WKW, Yu MS, Lai CSW, Yeung SC, et al. Effects of all-trans-retinoic acid on human SH-SY5Y neuroblastoma as in vitro model in neurotoxicity research. Neuro Toxicol 2009;30:127-35.
DOI: 10.1016/j.neuro.2008.11.001

Zhang L, Yu H, Sun Y, Lin X, Chen B, et al. Protective effects of salidroside on hydrogen peroxide-induced apoptosis in SH-SY5Y human neuroblastoma cells. Eur J Pharmacol 2007;564:18-25.
DOI: 10.1016/j.ejphar.2007.01.089

Gao M, Zhang WC, Liu QS, Hu JJ, Liu GT, et al. Pinocembrin prevents glutamate- induced apoptosis in SH-SY5Y neuronal cells via decrease of bax/bcl-2 ratio. Eur J Pharmacol 2008;591:73-9.
DOI: 10.1016/j.ejphar.2008.06.071

Veciana C, Cortés E, Torro L, Sirvent E, Rizo M, et al. Evaluación de la citotoxicidad y bioseguridad de un extracto de polifenoles de huesos de aceitunas. Nutr Hosp 2014;29:1388-93.

Kim HJ, Song JY, Park HJ, Park HK, Yun DH, et al. Naringin Protects against Rotenone-induced Apoptosis in Human Neuroblastoma SHSY5Y Cells. Kor J Physiol Pharmacol 2009;13:281-5.
DOI: 10.4196/kjpp.2009.13.4.281

Jaworska-Feil L, Jantas D, Leskiewicz M, Budziszewska B, Kubera M, et al. Protective effects of TRH and its analogues against various cytotoxic agents in retinoic acid (RA)-differentiated human neuroblastoma SH-SY5Y cells. Neuropeptides 2010;44:495-508.
DOI: 10.1016/j.npep.2010.08.004

Sears B, Ricordi C. Role of fatty acids and polyphenols in inflammatory gene transcription and their impact on obesity, metabolic syndrome and diabetes. Eur Rev Med Pharmacol Sci 2012;16:1137-54.

Commenges D. Intake of flavonoids and risk of dementia. Eur J Epidemiol 2000;16:357-63.
DOI: 10.1023/A:1007614613771

Gimenez-Cassina A, Lim F, Diaz-Nido J. Differentiation of a human neuroblastoma into neuron-like cells increases their susceptibility to transduction by herpesviral vectors. J Neurosci Res 2006;84:755-67.
DOI: 10.1002/jnr.20976

Lindahl PE, Oberg KE. The effect of rotenone on respiration and its point of attack. Exp Cell Res 1961;23:228-37.
DOI: 10.1016/0014-4827(61)90033-7

Oltavi ZN, Milliman CL, Korsmeyer SJ. Bcl-2 heterodimerizes in vivo with a conserved homolog, Bax, that accelerates programmed cell death. Cell 1993;74:609-19.
DOI: 10.1016/0092-8674(93)90509-O

Adams JM, Cory S. The Bcl-2 protein family: arbiters of cell survival. Science 1998;281:1322-6.
DOI: 10.1126/science.281.5381.1322

Schaffer S, Müller WE, Eckert GP. Cytoprotective effects of olive mill wastewater extract and its main constituent hydroxytyrosol in PC12 cells. Pharmacol Res 2010;62:322-7.
DOI: 10.1016/j.phrs.2010.06.004

Agudo M, Trejo JL, Lim F, Avila J, Torres-Aleman I, et al. Highly efficient and specific gene transfer to Purkinje cells in vivo using a herpes simplex virus I amplicon. Hum Gene Ther 2002;13:665-74.
DOI: 10.1089/10430340252837251

Morrison ME, Mason CA. Granule neuron regulation of Purkinje cell development: striking a balance between neurotrophin and glutamate signaling. J Neurosci 1998;18:3563-73.
DOI: 10.1523/JNEUROSCI.18-10-03563.1998

Bravin M, Morando L, Vercelli A, Rossi F, Strata P. Control of spine formation by electrical activity in the adult rat cerebellum. Proc Natl Acad Sci USA 1999;96:1704-9.
DOI: 10.1073/pnas.96.4.1704

Bijur GN, Jope RS. Glycogen synthase kinase-3beta is highly activated in nuclei and mitochondria. Neuroreport 2003;14:2415-9.
DOI: 10.1097/00001756-200312190-00025

Linseman DA, Butts BD, Precht TA, Phelps RA, Le SS, et al. Glycogen synthase kinase-3beta phosphorylates Bax and promotes its mitochondrial localization during neuronal apoptosis. J Neurosci 2004;24:9993-10002.
DOI: 10.1523/JNEUROSCI.2057-04.2004

Chao MV. Neurotrophins and their receptors: a convergence point for many signalling pathways. Nat Rev Neurosci 2003;4:299-309.
DOI: 10.1038/nrn1078

Perez-Navarro E, Gavalda N, Gratacos E, Alberch J. Brain-derived neurotrophic factor prevents changes in Bcl-2 family members and caspase-3 activation induced by excitotoxicity in the striatum. J Neurochem 2005;92:678-91.
DOI: 10.1111/j.1471-4159.2004.02904.x

Mohagheghi F, Bigdeli MR, Rasoulian B, Hashemi P, Pour MR neuroprotective effect of olive leaf extract is related to improved blood-brain barrier permeability and brain edema in rat with experimental focal cerebral ischemia. Phytomedicine 2011;18:170-5.

Dekanski D, Selakovi V, Piperski V, Radulovi Z, Koreni A, et al. Protective effect of olive leaf extract on hippocampal injury induced by transient global cerebral ischemia and reperfusion in Mongolian gerbils. Phytomedicine 2011;18:1137-43.
DOI: 10.1016/j.phymed.2011.05.010

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Trabajo Original

Protection by polyphenol extract from olive stones against apoptosis produced by oxidative stress in human neuroblastoma cells

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