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The administration of different polyphenols protects against increased body weight and fat accumulation. The aim of the study was to determine the anti-adipogenic activity of an olive-seed polyphenolic extract, by means of mouse fibroblast cell line 3T3-L1 adipocyte differentiation. Material and methods: cells were incubated and differentiated (6000 cells/cup) in the presence of olive-seed extract at 10 and 50 mg/l biosecure concentrations of polyphenols, and with no extract in the control sample. After 5 to 7 days mature adipocytes are formed. The fat clusters are quantified by means of red-oil staining, 490 nm absorbance, and the expression of the leptin and PPARg genes, and then compared to the values obtained in the cultures before and after adipocyte differentiation. Results: the control samples, with no extract, presented an accumulation of fat of 100%. By contrast, the addition of 50 mg/l of olive-seed extract polyphenols resulted in a 50% accumulation of fat, similar to that of the non-differentiated cells. A 10 mg/l extract concentration had no effect. Anti-adipogenic activity is thus confirmed, as the expression of the PPARg and leptin genes is reduced in adipocyte differentiation in the presence of extract at 50 mg/l. In conclusion, both the formation of fatty substances characteristic of adipogenesis, and the expression of the adipogenic PPARg and leptin genes are found to be inhibited by the prior addition of olive-seed extract polyphenols at a 50 mg/l concentration.
Palabras Clave: Adipogenesis. Fat accumulation. Inhibition. Olive-seed extract. Polyphenols.
Ernesto Cortés-Castell , Carmen Veciana-galindo , Luis Torró-montell , Antonio Palazón-Bru , Elia Sirvent-Segura , Vicente Gil-guillén , Mercedes Rizo-baeza
Isabel Moreno Indias
Jorge Carlos Ruíz Ruíz , Maira Rubi Segura-Campos
Tonancy Nicolás-Méndez , Alda Rocío Ortiz-Muñiz , Víctor Manuel Mendoza-Núñez , María del Carmen García-Rodríguez
Ayana Florencio de Meneses , Ana Luiza de Rezende Ferreira Mendes , Daianne Cristina Rocha , Helena Alves de Carvalho Sampaio , Antônio Augusto Ferreira Carioca , Luis Gonzaga Porto Pinheiro , Paulo Henrique Diógenes Vasques , Ilana Nogueira Bezerra , Leandro Teixeira Cacau
Carla Guzmán Pincheira , Fernanda Fierro Jara
Francisco José Tinahones Madueño
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