Nutrición Hospitalaria 03978 /
Resumen| PDF (ENGLISH)

Trabajo Original

Modulatory activity of a bovine hydrolyzed collagen-hydroxyapatite food complex on human primary osteoblasts after simulating its gastrointestinal digestion and absorption

Laura Soriano Romaní, Juan Antonio Nieto Fuentes, Lidia Tomás Cobos, Elena Diez Sánchez

Logo Descargas   Número de descargas: 63      Logo Visitas   Número de visitas: 187      Citas   Citas: 0


Introduction: osteoporosis is the most prevalent bone disease and one of the main causes of chronic disability in middle and advanced ages. Conventional pharmacological treatments are still limited, and their prolonged use can cause adverse effects that motivate poor adherence to treatment. Nutritional strategies are traditionally based on supplementing the diet with calcium and vitamin D. Recent studies confirm that the results of this supplementation are significantly improved if it is accompanied by the intake of oral hydrolyzed collagen. Objective: to evaluate the possible in vitro osteogenic activity of a peptide-mineral complex formed by bovine hydrolyzed collagen and bovine hydroxyapatite (Phoscollagen®, PHC®). Methods: the digestion and absorption of PHC® were simulated using the dynamic gastrointestinal digester of AINIA and Caco-2 cell model, respectively. Primary cultures of human osteoblasts were treated with the resulting fraction of PHC® and changes were evaluated in the proliferation of preosteoblasts and in the mRNA expression of osteogenic biomarkers at different stages of osteoblast maturation: Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteocalcin (OC) and type I collagen (ColA1). Results: an increase in preosteoblastic proliferation was observed (p ≤ 0,05). No changes were detected in the biomarkers of osteoblasts with 5 days of differentiation, but were with 14 days, registering an increase in Runx2 (p = 0.0008), ColA1 (p = 0.035), OC (p = 0.027) and ALP (without significance). Conclusion: these results show that the PHC® peptide-mineral complex stimulates the activity of mature osteoblasts, being capable of promoting bone formation.

Palabras Clave: Digestion. Hydrolyzed collagen. Hydroxyapatite. Osteoblasts. Osteoporosis. Peptides.

Odén, A.; McCloskey, E. V.; Kanis, J.A.; Harvey, N.C.; Johansson, H. Burden of high fracture probability worldwide: secular increases 2010-2040. Osteoporos. Int.2015, 26, 2243–2248,
Sozen, T.; Ozisik, L.; Calik Basaran, N. An overview and management of osteoporosis. Eur. J. Rheumatol.2017, 4, 46–56,
Mücke, T.; Krestan, C.R.; Mitchell, D.A.; Kirschke, J.S.; Wutzl, A. Bisphosphonate and medication-related osteonecrosis of the jaw: A review. Semin. Musculoskelet. Radiol.2016, 20, 305–314,
Viguet-Carrin, S.; Garnero, P.; Delmas, P.D. The role of collagen in bone strength. Osteoporos. Int.2006, 17, 319–336,
Sadat-Shojai, M.; Khorasani, M.T.; Dinpanah-Khoshdargi, E.; Jamshidi, A. Synthesis methods for nanosized hydroxyapatite with diverse structures. Acta Biomater.2013, 9, 7591–7621,
Aurégan, J.C.; Bosser, C.; Bensidhoum, M.; Bégué, T.; Hoc, T. Correlation between skin and bone parameters in women with postmenopausal osteoporosis: A systematic review. EFORT open Rev.2018, 3, 449–460,
Weaver, C.M.; Alexander, D.D.; Boushey, C.J.; Dawson-Hughes, B.; Lappe, J.M.; LeBoff, M.S.; Liu, S.; Looker, A.C.; Wallace, T.C.; Wang, D.D. Calcium plus vitamin D supplementation and risk of fractures: an updated meta-analysis from the National Osteoporosis Foundation. Osteoporos. Int.2016, 27, 367–376,
Liu, C.; Kuang, X.; Li, K.; Guo, X.; Deng, Q.; Li, D. Effects of combined calcium and vitamin D supplementation on osteoporosis in postmenopausal women: a systematic review and meta-analysis of randomized controlled trials. Food Funct.2020, 11, 10817–10827,
Zhao, L.; Li, M.; Sun, H. Effects of dietary calcium to available phosphorus ratios on bone metabolism and osteoclast activity of the OPG /RANK/RANKL signalling pathway in piglets. J. Anim. Physiol. Anim. Nutr. (Berl).2019, 103, 1224–1232,
Sun, X.; Sarteshnizi, R.A.; Boachie, R.T.; Okagu, O.D.; Abioye, R.O.; Neves, R.P.; Ohanenye, I.C.; Udenigwe, C.C. Peptide-Mineral Complexes: Understanding Their Chemical Interactions, Bioavailability, and Potential Application in Mitigating Micronutrient Deficiency. Foods (Basel, Switzerland)2020, 9,
DOI: 10.3390/foods9101402
Epstein, O.; Kato, Y.; Dick, R.; Sherlock, S. Vitamin D, hydroxyapatite, and calcium gluconate in treatment of cortical bone thinning in postmenopausal women with primary biliary cirrhosis. Am. J. Clin. Nutr.1982, 36, 426–430,
Castelo-Branco, C.; Cancelo Hidalgo, M.J.; Palacios, S.; Ciria-Recasens, M.; Fernández-Pareja, A.; Carbonell-Abella, C.; Manasanch, J.; Haya-Palazuelos, J. Efficacy and safety of ossein-hydroxyapatite complex versus calcium carbonate to prevent bone loss. Climacteric2020, 23, 252–258,
DOI: 10.1080/13697137.2019.1685488
Shigemura, Y.; Suzuki, A.; Kurokawa, M.; Sato, Y.; Sato, K. Changes in composition and content of food-derived peptide in human blood after daily ingestion of collagen hydrolysate for 4 weeks. J. Sci. Food Agric.2018, 98, 1944–1950,
Zhang, K.; Li, B.; Chen, Q.; Zhang, Z.; Zhao, X.; Hou, H. Functional calcium binding peptides from pacific cod (Gadus macrocephalus) bone: Calcium bioavailability enhancing activity and anti-osteoporosis effects in the ovariectomy-induced osteoporosis rat model. Nutrients2018, 10,
DOI: 10.3390/nu10091325
Chen, M.; Li, Y.; Huang, G. Potential Health Functions of Collagen Bioactive Peptides: A Review. Am. J. Biochem. Biotechnol.2020, 16, 507–519,
Kim, H.K.; Kim, M.G.; Leem, K.H. Collagen hydrolysates increased osteogenic gene expressions via a MAPK signaling pathway in MG-63 human osteoblasts. Food Funct.2014, 5, 573–578,
Ye, M.; Zhang, C.; Zhu, L.; Jia, W.; Shen, Q. Yak (Bos grunniens) bones collagen-derived peptides stimulate osteoblastic proliferation and differentiation via the activation of Wnt/β-catenin signaling pathway. J. Sci. Food Agric.2020, 100, 2600–2609,
Porfírio, E.; Fanaro, G.B. Collagen supplementation as a complementary therapy for the prevention and treatment of osteoporosis and osteoarthritis: a systematic review. Rev. Bras. Geriatr. e Gerontol.2016, 19, 153–164,
DOI: 10.1590/1809-9823.2016.14145
Daneault, A.; Prawitt, J.; Fabien Soulé, V.; Coxam, V.; Wittrant, Y. Biological effect of hydrolyzed collagen on bone metabolism. Crit. Rev. Food Sci. Nutr.2017, 57, 1922–1937,
Elam, M.L.; Hooshmand, S.; Browne, J.; Campbell, S.C.; Payton, M.E.; Gu, J.; Arjmandi, B.H. Evidence for bone reversal properties of a calcium-collagen chelate, a novel dietary supplement. researchgate.net2013, 2, 1,
Elam, M.L.; Johnson, S.A.; Hooshmand, S.; Feresin, R.G.; Payton, M.E.; Gu, J.; Arjmandi, B.H. A calcium-collagen chelate dietary supplement attenuates bone loss in postmenopausal women with osteopenia: a randomized controlled trial. J. Med. Food2015, 18, 324–331,
DOI: 10.1089/jmf.2014.0100
Argyrou, C.; Karlafti, E.; Lampropoulou-Adamidou, K.; Tournis, S.; Makris, K.; Trovas, G.; Dontas, I.; Triantafyllopoulos, I.K. Effect of calcium and vitamin D supplementation with and without collagen peptides on bone turnover in postmenopausal women with osteopenia. J. Musculoskelet. Neuronal Interact.2020, 20, 12.
Martin-Bautista, E.; Martin-Matillas, M.; Martin-Lagos, J.A.; Miranda-Leon, M.T.; Muñoz-Torres, M.; Ruiz-Requena, E.; Rivero, M.; Quer, J.; Puigdueta, I.; Campoy, C. A nutritional intervention study with hydrolyzed collagen in pre-pubertal spanish children: influence on bone modeling biomarkers. J. Pediatr. Endocrinol. Metab.2011, 24, 147–153,
Cúneo, F.; Costa-Paiva, L.; Pinto-Neto, A.M.; Morais, S.S.; Amaya-Farfan, J. Effect of dietary supplementation with collagen hydrolysates on bone metabolism of postmenopausal women with low mineral density. Maturitas2010, 65, 253–257,
DOI: 10.1016/j.maturitas.2009.10.002
Nieto, J.; Soriano-Romaní, L.; Tomás-Cobos, L.; Sharma, L.; Budde, T. Improved in vitro bioavailability of a newly developed functionalized calcium carbonate salt as a food ingredient and its comparison with available commercial calcium salts. Food Chem.2021, 348,
DOI: 10.1016/j.foodchem.2020.128740
Hemery, Y.M.; Anson, N.M.; Havenaar, R.; Haenen, G.R.M.M.; Noort, M.W.J.; Rouau, X. Dry-fractionation of wheat bran increases the bioaccessibility of phenolic acids in breads made from processed bran fractions. Food Res. Int.2010, 43, 1429–1438,
Blanquet, S.; Zeijdner, E.; Beyssac, E.; Meunier, J.-P.; Denis, S.; Havenaar, R.; Alric, M. A dynamic artificial gastrointestinal system for studying the behavior of orally administered drug dosage forms under various physiological conditions. Pharm. Res.2004, 21, 585–591.
Hubatsch, I.; Ragnarsson, E.G.E.; Artursson, P. Determination of drug permeability and prediction of drug absorption in Caco-2 monolayers. Nat. Protoc.2007, 2, 2111–2119,
Wu, W.; He, L.; Liang, Y.; Yue, L.; Peng, W.; Jin, G.; Ma, M. Preparation process optimization of pig bone collagen peptide-calcium chelate using response surface methodology and its structural characterization and stability analysis. Food Chem.2019, 284, 80–89,
Rubert, M.; de la Piedra, C. Osteocalcin: From marker of bone formation to hormone; and bone, an endocrine organ. Rev. Osteoporos. y Metab. Miner.2021, 12, 146–151,
DOI: 10.4321/S1889-836X2020000400007
Ye, M.; Zhang, C.; Jia, W.; Shen, Q.; Qin, X.; Zhang, H.; Zhu, L. Metabolomics strategy reveals the osteogenic mechanism of yak (Bos grunniens) bone collagen peptides on ovariectomy-induced osteoporosis in rats. Food Funct.2020, 11, 1498–1512,
Woo, M.; Noh, J.S. Regulatory Effects of Skate Skin-Derived Collagen Peptides with Different Molecular Weights on Lipid Metabolism in the Liver and Adipose Tissue. Biomedicines2020, 8,
DOI: 10.3390/biomedicines8070187
Zhu, C.F.; Li, G.Z.; Peng, H. Bin; Zhang, F.; Chen, Y.; Li, Y. Treatment with marine collagen peptides modulates glucose and lipid metabolism in Chinese patients with type 2 diabetes mellitus. Appl. Physiol. Nutr. Metab.2010, 35, 797–804,
Fu, Y.; Zhao, X.H. In vitro responses of hFOB1.19 cells towards chum salmon (Oncorhynchus keta) skin gelatin hydrolysates in cell proliferation, cycle progression and apoptosis. J. Funct. Foods2013, 5, 279–288,
DOI: 10.1016/j.jff.2012.10.017
Liu, J.L.; Zhang, B.; Song, S.J.; Ma, M.; Si, S.Y.; Wang, Y.H.; Xu, B.X.; Feng, K.; Wu, J.G.; Guo, Y.C. Bovine collagen peptides compounds promote the proliferation and differentiation of MC3T3-E1 pre-osteoblasts. PLoS One2014, 9, 99920,
DOI: 10.1371/journal.pone.0099920
Zhu, L.; Xie, Y.; Wen, B.; Ye, M.; Liu, Y.; Imam, K.M.S.U.; Cai, H.; Zhang, C.; Wang, F.; Xin, F. Porcine bone collagen peptides promote osteoblast proliferation and differentiation by activating the PI3K/Akt signaling pathway. J. Funct. Foods2020, 64, 103697,
DOI: 10.1016/j.jff.2019.103697
Wu, W.; He, L.; Li, C.; Zhao, S.; Liang, Y.; Yang, F.; Zhang, M.; Jin, G.; Ma, M. Phosphorylation of porcine bone collagen peptide to improve its calcium chelating capacity and its effect on promoting the proliferation, differentiation and mineralization of osteoblastic MC3T3-E1 cells. J. Funct. Foods2020, 64, 103701,
DOI: 10.1016/j.jff.2019.103701
Song, W.; Chen, Q.; Wang, Y.; Han, Y.; Zhang, H.; Li, B.; Yu, G. Identification and Structure–Activity Relationship of Intestinal Epithelial Barrier Function Protective Collagen Peptides from Alaska Pollock Skin. Mar. Drugs2019, 17,
DOI: 10.3390/md17080450
Wauquier, F.; Daneault, A.; Granel, H.; Prawitt, J.; Soulé, V.F.; Berger, J.; Pereira, B.; Guicheux, J.; Rochefort, G.Y.; Meunier, N.; et al. Human enriched serum following hydrolysed collagen absorption modulates bone cell activity: From bedside to bench and vice versa. Nutrients2019, 11,
DOI: 10.3390/nu11061249
Kim, H.K.; Kim, M.G.; Leem, K.H. Osteogenic activity of collagen peptide via ERK/MAPK pathway mediated boosting of collagen synthesis and its therapeutic efficacy in osteoporotic bone by back-scattered electron imaging and microarchitecture analysis. Molecules2013, 18, 15474–15489,
DOI: 10.3390/molecules181215474

Carta Editor: RÉplica: “revisiÓn de los efectos beneficiosos de la ingesta de colÁgeno hidrolizado sobre la salud osteoarticular y el envejecimiento dÉrmico”

José Miguel Senabre Gallego , Esteban Salas Heredia , Gregorio Santos Soler , José Rosas

Publicado: 2016-02-17 /

Artículo Especial: El calcio, esencial para la salud

Emilio Martínez De Victoria

Publicado: 2016-07-12 /

Trabajo Original: Efecto cicatrizante de fracciones peptídicas derivadas de la hidrólisis enzimática de proteínas de Phaseolus lunatus

Ruth Lezama García , Luis Chel Guerrero , Juan José Acevedo Fernández , David Betancur Ancona

Publicado: 2018-04-05 /

Trabajo Original: Casein-derived peptides as an alternative ingredient for low-phenylalanine diets

Claudia C.H. Krüger , Thaise D. Azevedo , Marina Talamini Piltz , Ágatha Transfeld Silva , Lys Mary Bileski Cândido

Publicado: 2018-11-04 /

Trabajo Original: Actividad física y estilo de vida relacionado con la salud en la población española con enfermedad musculoesquelética

Jesús García Mayor , Antonio Moreno Llamas , Ernesto de la Cruz Sánchez

Publicado: 2020-01-09 /

Trabajo Original: Post-menopausal osteoporosis: do body composition, nutritional habits, and physical activity affect bone mineral density?

Özlem Özpak Akkuş , Betül Atalay

Publicado: 2020-06-13 /

Trabajo Original: Nutrición en la prevención y el control de la osteoporosis

Rosa M. Ortega Anta , Ana Isabel Jiménez Ortega , Rosa María Martínez García , Esther Cuadrado Soto , Aránzazu Aparicio , Ana M. López-Sobaler

Publicado: 2020-09-22 /

Revisión: Envejecimiento y composición corporal: la obesidad sarcopénica en España

Publicado: 2021-09-28 /

Trabajo Original: Analysis of bone microarchitecture related to anthropometry in climateric women

Publicado: 2021-09-28 /

Trabajo Original: Asociación del polimorfismo TaqI del gen del receptor de la vitamina D con la densidad mineral ósea en mujeres mexicanas jóvenes

Publicado: 2021-10-04 /

Trabajo Original: Expectativas del beneficio/riesgo de la ingesta de calcio en mujeres con tratamientos para osteoporosis de Asturias; estudio ASFARCAL

Publicado: 2021-10-19 /

Trabajo Original: Disminución de masa ósea post-cirugía bariátrica con by-pass en Y de Roux

Publicado: 2021-10-20 /

Revisión: Energy availability, menstrual dysfunction and bone health in sports; an overwiew of the female athlete triad

Publicado: 2021-10-26 /

Trabajo Original: Do calcium and vitamin D intake influence the effect of cycling on bone mass through adolescence?

Publicado: 2021-10-26 /

Revisión: Compuestos bioactivos de la dieta con potencial en la prevención de patologías relacionadas con sobrepeso y obesidad; péptidos biológicamente activos

Publicado: 2021-11-02 /

Trabajo Original: The high glycemic index diet was an independent predictor to explain changes in agouti-related protein in obese adolescents

Publicado: 2021-11-02 /

Trabajo Original: Association between magnesium-deficient status and anthropometric and clinical-nutritional parameters in posmenopausal women

Publicado: 2021-11-03 /

Revisión: Bioactive vegetable proteins and peptides in lipid-lowering; nutraceutical potential

Publicado: 2021-11-03 /

Revisión: Adherence to Mediterranean diet and bone health

Publicado: 2021-11-03 /

Trabajo Original: Densidad mineral ósea, calcio dietético y factores presuntivos de riesgo de osteoporosis en mujeres ecuatorianas de la tercera edad

Publicado: 2021-11-04 /

Trabajo Original: Swimming training repercussion on metabolic and structural bone development; benefits of the incorporation of whole body vibration or pilometric training; the RENACIMIENTO project

Publicado: 2021-11-04 /

Trabajo Original: Effects of eight months of whole body vibration training on hip bone mass in older women

Publicado: 2021-11-26 /

Trabajo Original: Los países mediterráneos ante la dieta mediterránea, ¿seguimos en el buen camino? El ejemplo de las mujeres de mediana edad del sur de España

Publicado: 2021-11-30 /

Trabajo Original: Osteoporosis e índice de masa corporal en el trasplantado renal

Publicado: 2022-01-12 /

Trabajo Original: Estudios de inhibición in vitro de la enzima convertidora de angiotensina-I, efectos hipotensor y antihipertensivo de fracciones peptídicas de V. unguiculata

Publicado: 2022-01-20 /

Trabajo Original: Lactancia materna como factor preventivo para la osteoporosis en mujeres adultas

Publicado: 2022-01-21 /

Artículos más populares

Trabajo Original: Vitamina D y componentes del síndrome metabólico en candidatos a cirugía bariátrica

Objetivo: el objetivo de este estudio fue evaluar...

Publicado: 2016-02-16

Revisión: Papel de la nutrición en la recuperación del jugador de baloncesto

Introducción: son escasos los trabajos que ofrecen...

Publicado: 2019-03-11

Trabajo Original: Hábitos alimentarios en la población gallega durante el confinamiento por la COVID-19

Introducción: en plena crisis por la COVID-19, el...

Publicado: 2020-06-12

Una cookie o galleta informática es un pequeño archivo de información que se guarda en su navegador cada vez que visita nuestra página web. La utilidad de las cookies es guardar el historial de su actividad en nuestra página web, de manera que, cuando la visite nuevamente, ésta pueda identificarle y configurar el contenido de la misma en base a sus hábitos de navegación, identidad y preferencias. Las cookies pueden ser aceptadas, rechazadas, bloqueadas y borradas, según desee. Ello podrá hacerlo mediante las opciones disponibles en la presente ventana o a través de la configuración de su navegador, según el caso. En caso de que rechace las cookies no podremos asegurarle el correcto funcionamiento de las distintas funcionalidades de nuestra página web. Más información en el apartado “POLÍTICA DE COOKIES” de nuestra página web.