Laura Soriano Romaní, Juan Antonio Nieto Fuentes, Lidia Tomás Cobos, Elena Diez Sánchez
Número de descargas:
589
Número de visitas:
1996
Compártelo:
Introduction: osteoporosis is the most prevalent bone disease and one of the main causes of chronic disability in middle and advanced ages. Conventional pharmacological treatments are still limited, and their prolonged use can cause adverse effects that motivate poor adherence to treatment. Nutritional strategies are traditionally based on supplementing the diet with calcium and vitamin D. Recent studies confirm that the results of this supplementation are significantly improved if it is accompanied by the intake of oral hydrolyzed collagen. Objective: to evaluate the possible in vitro osteogenic activity of a peptide-mineral complex formed by bovine hydrolyzed collagen and bovine hydroxyapatite (Phoscollagen®, PHC®). Methods: the digestion and absorption of PHC® were simulated using the dynamic gastrointestinal digester of AINIA and Caco-2 cell model, respectively. Primary cultures of human osteoblasts were treated with the resulting fraction of PHC® and changes were evaluated in the proliferation of preosteoblasts and in the mRNA expression of osteogenic biomarkers at different stages of osteoblast maturation: Runt-related transcription factor 2 (Runx2), alkaline phosphatase (ALP), osteocalcin (OC) and type I collagen (ColA1). Results: an increase in preosteoblastic proliferation was observed (p ≤ 0,05). No changes were detected in the biomarkers of osteoblasts with 5 days of differentiation, but were with 14 days, registering an increase in Runx2 (p = 0.0008), ColA1 (p = 0.035), OC (p = 0.027) and ALP (without significance). Conclusion: these results show that the PHC® peptide-mineral complex stimulates the activity of mature osteoblasts, being capable of promoting bone formation.
Palabras Clave: Digestion. Hydrolyzed collagen. Hydroxyapatite. Osteoblasts. Osteoporosis. Peptides.
José Miguel Senabre Gallego , Esteban Salas Heredia , Gregorio Santos Soler , José Rosas
Emilio Martínez De Victoria
Ruth Lezama García , Luis Chel Guerrero , Juan José Acevedo Fernández , David Betancur Ancona
Claudia C.H. Krüger , Thaise D. Azevedo , Marina Talamini Piltz , Ágatha Transfeld Silva , Lys Mary Bileski Cândido
Jesús García Mayor , Antonio Moreno Llamas , Ernesto de la Cruz Sánchez
Özlem Özpak Akkuş , Betül Atalay
Rosa M. Ortega , Ana Isabel Jiménez Ortega , Rosa María Martínez García , Esther Cuadrado Soto , Aránzazu Aparicio , Ana M. López-Sobaler
Diana Montiel-Ojeda , Sonia Cerdas , Patricia Clark , Mónica Caló , Sofía Wullich , Ramiro Da Silva Llibre , Joan Levin Ford
Teresa Sánchez-Moya , Siham Ydjedd , Carmen Frontela-Saseta , Rubén López Nicolás , Gaspar Ros-Berruezo
Alejandro Sanz Paris , Ana Artero , Rosa Burgos Peláez , Jose Manuel García Almeida , Pilar Matía Martín , Samara Palma Milla , Ana Zugasti Murillo , José Joaquín Alfaro Martínez , Alfonso Calañas Continente , M.ª Jesús Chinchetru , Katherine García Malpartida , Ángela González-Díaz Faes , Víctor González-Sánchez , María Laínez López , Antonio Jesús Martínez Ortega , Juana Oliva Roldán , José Pablo Suárez Llanos , Clara Serrano-Moreno
Background: overweight and obesity have been incre...
Objetivo: el presente estudio tiene como objetivo...