Nutrición Hospitalaria 04366 / http://dx.doi.org/10.20960/nh.04366

Trabajo Original

In vitro prebiotic activity of rhLf and galactooligosaccharides on infant intestinal microbiota

Esmat Aly, Gaspar Ros-Berruezo, Rubén López Nicolás, Aliaa Ali Darwish, Teresa Sánchez-Moya, David Planes-Muñoz, Carmen Frontela-Saseta

Prepublicado: 2023-04-18

Publicado: 2023-07-28

VOLUMEN 40, NÚM. 4, julio-agosto (2023), PAG. 701-710

Logo Descargas Número de descargas: 115 Logo Visitas Número de visitas: 1392 Citas Citas: 0

Compártelo:

Objective: human lactoferrin (Lf) and human milk oligosaccharides possess a wide range of functions. So, the present study focusses on the role of Lf and/or galactooligosaccharides (GOS) in the modulation of gut microbiota composition. Methods: recombinant human lactoferrin (rhLf) was added to the first infant formula (0.10, 0.15, 0.20 %) alone or in combination with GOS (1 %) in vessels of a small-scale batch culture fermentation model. Short-chain fatty acids (SCFAs), microbial population groups, and pH were monitored through fermentation for 24 hours. Results: insignificant changes were observed in pH values and acetic acid accumulated during fermentation. Propionic acid content has been insignificantly increased while butyric acid has been insignificantly decreased. Moreover, increments in all bacterial groups except for Bacteroides were observed through the fermentation process. Lactobacillus and Bifidobacterium showed an increase in relation to initial time over the fermentation process, demonstrating the prebiotic effect of lactoferrin and GOS. After 24 hours of fermentation, all tested ingredients showed significant similarities in Enterococcus for controls except for 0.20 % rhLf + 1 % GOS, which provoked a diminution of Enterococci growth. Conclusion: despite the importance of the batch culture fermentation technique in uncovering the prebiotic activity of food ingredients, it is not useful for detecting the prebiotic nature of Lf due to its nature as a protein. Thus, Lf maybe shows its prebiotic activity on the gut microbiota through other mechanisms.

Palabras Clave: Lactoferrin. Galactooligosaccharides. Intestinal microbiota. Prebiotic activity.

Descargar PDF (ENGLISH)

Bibliografía
Artículos Relacionados

Saarela M, Lahteenmaki L, Crittenden R, Salminen S, Mattila-Sandholm T. Gut bacteria and health foods - the European perspective. International Journal of Food Microbiology. 2002;78(1-2):99-117.
DOI: 10.1016/S0168-1605(02)00235-0

Orsi N. The antimicrobial activity of lactoferrin: Current status and perspectives. Biometals. 2004;17(3):189-96.
DOI: 10.1023/B:BIOM.0000027691.86757.e2

Mastromarino P, Capobianco D, Campagna G, Laforgia N, Drimaco P, Dileone A, et al. Correlation between lactoferrin and beneficial microbiota in breast milk and infant's feces. Biometals. 2014;27(5):1077-86.
DOI: 10.1007/s10534-014-9762-3

Oda H, Wakabayashi H, Yamauchi K, Abe F. Lactoferrin and bifidobacteria. Biometals. 2014;27(5):915-22.
DOI: 10.1007/s10534-014-9741-8

Granier A, Goulet O, Hoarau C. Fermentation products: immunological effects on human and animal models. Pediatric Research. 2013;74(2):238-44.
DOI: 10.1038/pr.2013.76

Aly E, López-Nicolás R, Darwish AA, Frontela-Saseta C, Ros-Berruezo G. Supplementation of infant formulas with recombinant human lactoferrin and/or galactooligosaccharides increases iron bioaccessibility as measured by ferritin formed in Caco-2 cell model. Food Research International. 2016;89:1048-55.
DOI: 10.1016/j.foodres.2016.08.030

Sanchez-Moya T, Lopez-Nicolas R, Planes D, Gonzalez-Bermudez CA, Ros-Berruezo G, Frontela-Saseta C. In vitro modulation of gut microbiota by whey protein to preserve intestinal health. Food & Function. 2017;8(9):3053-63.
DOI: 10.1039/C7FO00197E

Beards E, Tuohy K, Gibson G. Bacterial, SCFA and gas profiles of a range of food ingredients following in vitro fermentation by human colonic microbiota. Anaerobe. 2010;16(4):420-5.
DOI: 10.1016/j.anaerobe.2010.05.006

Gonzalez-Bermudez CA, Lopez-Nicolas R, Peso-Echarri P, Fronteta-Saseta C, Martinez-Gracia C. Effects of different thickening agents on infant gut microbiota. Food & Function. 2018;9(3):1768-78.
DOI: 10.1039/C7FO01992K

Collado MC, Isolauri E, Laitinen K, Salminen S. Effect of mother's weight on infant's microbiota acquisition, composition, and activity during early infancy a prospective follow-up study initiated in early pregnancy. American Journal of Clinical Nutrition. 2010;92(5):1023-30.
DOI: 10.3945/ajcn.2010.29877

Farnaud S, Evans RW. Lactoferrin—a multifunctional protein with antimicrobial properties. Molecular Immunology. 2003;40(7):395-405.
DOI: 10.1016/S0161-5890(03)00152-4

Duncan SH, Louis P, Thomson JM, Flint HJ. The role of pH in determining the species composition of the human colonic microbiota. Environ Microbiol. 2009;11(8):2112-22. Epub 20090421.
DOI: 10.1111/j.1462-2920.2009.01931.x

Rycroft CE, Jones MR, Gibson GR, Rastall RA. A comparative in vitro evaluation of the fermentation properties of prebiotic oligosaccharides. Journal of Applied Microbiology. 2001;91(5):878-87.
DOI: 10.1046/j.1365-2672.2001.01446.x

den Besten G, van Eunen K, Groen AK, Venema K, Reijngoud DJ, Bakker BM. The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism. J Lipid Res. 2013;54(9):2325-40. Epub 20130702.
DOI: 10.1194/jlr.R036012

Bougle D, Vaghefi-Vaezzadeh N, Roland N, Bouvard G, Arhan P, Bureau F, et al. Influence of short-chain fatty acids on iron absorption by proximal colon. Scandinavian Journal of Gastroenterology. 2002;37(9):1008-11.
DOI: 10.1080/003655202320378176

Miller TL, Wolin MJ. Pathways of acetate, propionate, and butyrate formation by the human fecal microbial flora. Applied and Environmental Microbiology. 1996;62(5):1589-92. PubMed PMID: WOS:A1996UJ22800016.
DOI: 10.1128/aem.62.5.1589-1592.1996

Velazquez M, Davies C, Marett R, Slavin JL, Feirtag JM. Effect of oligosaccharides and fibre substitutes on short-chain fatty acid production by human faecal microflora. Anaerobe. 2000;6(2):87-92.
DOI: 10.1006/anae.1999.0318

Cardelle-Cobas A, Fernandez M, Salazar N, Martinez-Villaluenga C, Villamiel M, Ruas-Madiedo P, et al. Bifidogenic effect and stimulation of short chain fatty acid production in human faecal slurry cultures by oligosaccharides derived from lactose and lactulose. Journal of Dairy Research. 2009;76(3):317-25.
DOI: 10.1017/S0022029909004063

Knol J, Scholtens P, Kafka C, Steenbakkers J, Gross S, Helm K, et al. Colon microflora in infants fed formula with galacto- and fructo-oligosaccharides: More like breast-fed infants. Journal of Pediatric Gastroenterology and Nutrition. 2005;40(1):36-42.
DOI: 10.1097/00005176-200501000-00007

Fukuda S, Toh H, Hase K, Oshima K, Nakanishi Y, Yoshimura K, et al. Bifidobacteria can protect from enteropathogenic infection through production of acetate. Nature. 2011;469(7331):543-U791.
DOI: 10.1038/nature09646

Rodriguez-Colinas B, Kolida S, Baran M, Ballesteros AO, Rastall RA, Plou FJ. Analysis of fermentation selectivity of purified galacto-oligosaccharides by in vitro human faecal fermentation. Applied Microbiology and Biotechnology. 2013;97(13):5743-52.
DOI: 10.1007/s00253-013-4892-z

Walker AW, Duncan SH, Leitch ECM, Child MW, Flint HJ. pH and peptide supply can radically alter bacterial populations and short-chain fatty acid ratios within microbial communities from the human colon. Applied and Environmental Microbiology. 2005;71(7):3692-700.
DOI: 10.1128/AEM.71.7.3692-3700.2005

Al-Tamimi M, Palframan RJ, Cooper JM, Gibson GR, Rastall RA. In vitro fermentation of sugar beet arabinan and arabino-oligosaccharides by the human gut microflora. Journal of Applied Microbiology. 2006;100(2):407-14.
DOI: 10.1111/j.1365-2672.2005.02780.x

Rossi M, Corradini C, Amaretti A, Nicolini M, Pompei A, Zanoni S, et al. Fermentation of fructooligosaccharides and inulin by bifidobacteria: a comparative study of pure and fecal cultures. Applied and Environmental Microbiology. 2005;71(10):6150-8.
DOI: 10.1128/AEM.71.10.6150-6158.2005

Delzenne NM, Kok N. Effects of fructans-type prebiotics on lipid metabolism. American Journal of Clinical Nutrition. 2001;73(2):456S-8S. PubMed PMID: WOS:000166608200016.
DOI: 10.1093/ajcn/73.2.456s

Boehm G, Moro G. Structural and functional aspects of prebiotics used in infant nutrition. Journal of Nutrition. 2008;138(9):1818S-28S. PubMed PMID: WOS:000258802000040.
DOI: 10.1093/jn/138.9.1818S

Wong JMW, de Souza R, Kendall CWC, Emam A, Jenkins DJA. Colonic health: Fermentation and short chain fatty acids. Journal of Clinical Gastroenterology. 2006;40(3):235-43.
DOI: 10.1097/00004836-200603000-00015

Yeung CK, Glahn RP, Welch RM, Miller DD. Prebiotics and iron Bioavailability - Is there a connection? Journal of Food Science. 2005;70(5):R88-R92.
DOI: 10.1111/j.1365-2621.2005.tb09984.x

Weiss G. Iron metabolism in the anemia of chronic disease. Biochimica et Biophysica Acta. 2009; 1790: 682-693.
DOI: 10.1016/j.bbagen.2008.08.006

Lee YK, Mehrabian P, Boyajian S, Wu WL, Selicha J, Vonderfecht S, et al. The Protective Role of Bacteroides fragilis in Murine Model of Colitis-Associated Colorectal Cancer. Msphere. 2018;3(6).
DOI: 10.1128/mSphere.00587-18

Walker AW, Ince J, Duncan SH, Webster LM, Holtrop G, Ze X, et al. Dominant and diet-responsive groups of bacteria within the human colonic microbiota. Isme Journal. 2011;5(2):220-30.
DOI: 10.1038/ismej.2010.118

Serino M. Molecular Paths Linking Metabolic Diseases, Gut Microbiota Dysbiosis and Enterobacteria Infections. Journal of Molecular Biology. 2018;430(5):581-90.
DOI: 10.1016/j.jmb.2018.01.010

Murdock CA, Cleveland J, Matthews KR, Chikindas ML. The synergistic effect of nisin and lactoferrin on the inhibition of Listeria monocytogenes and Escherichia coli O157 : H7. Letters in Applied Microbiology. 2007;44(3):255-61.
DOI: 10.1111/j.1472-765X.2006.02076.x

Sun J, Ren FZ, Xiong L, Zhao L, Guo HY. Bovine lactoferrin suppresses high-fat diet induced obesity and modulates gut microbiota in C57BL/6J mice. Journal of Functional Foods. 2016;22:189-200.
DOI: 10.1016/j.jff.2016.01.022

Hernot DC, Boileau TW, Bauer LL, Middelbos IS, Murphy MR, Swanson KS, et al. In Vitro Fermentation Profiles, Gas Production Rates, and Microbiota Modulation as Affected by Certain Fructans, Galactooligosaccharides, and Polydextrose. Journal of Agricultural and Food Chemistry. 2009;57(4):1354-61.
DOI: 10.1021/jf802484j

Davis LMG, Martinez I, Walter J, Hutkins R. A dose dependent impact of prebiotic galactooligosaccharides on the intestinal microbiota of healthy adults. International Journal of Food Microbiology. 2010;144(2):285-92.
DOI: 10.1016/j.ijfoodmicro.2010.10.007

Hanchi H, Mottawea W, Sebei K, Hammami R. The Genus Enterococcus: Between Probiotic Potential and Safety Concerns-An Update. Frontiers in Microbiology. 2018;9.
DOI: 10.3389/fmicb.2018.01791

Li W, Wang KQ, Sun Y, Ye H, Hu B, Zeng XX. Influences of structures of galactooligosaccharides and fructooligosaccharides on the fermentation in vitro by human intestinal microbiota. Journal of Functional Foods. 2015;13:158-68.
DOI: 10.1016/j.jff.2014.12.044

Tian H, Maddox IS, Ferguson LR, Shu Q. Influence of bovine lactoferrin on selected probiotic bacteria and intestinal pathogens. Biometals. 2010;23(3):593-6.
DOI: 10.1007/s10534-010-9318-0

Artículos más populares

Revisión: Ayuno intermitente: efectos en diversos escenarios clínicos

Introducción: los esquemas de ayuno intermitente (...

Publicado: 2023-05-24

Resumen | PDF

Trabajo Original: Body mass index and risk of inflammatory breast disease: a Mendelian randomization study

Introduction: in previous studies, obesity was ide...

Publicado: 2023-04-22

Resumen | PDF

Revisión: Relación entre la dieta, aspectos nutricionales y la calidad del sueño en población pediátrica

.La relación entre la dieta y el sueño ha sido esc...

Publicado: 2023-06-08

Resumen | PDF

Licencia creative commons: Open Access bajo la licencia Creative Commons 4.0 CC BY-NC-SA
https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode

Editado en Madrid por:

Diseño y Desarrollo web Im3diA comunicación

Trabajo Original

In vitro prebiotic activity of rhLf and galactooligosaccharides on infant intestinal microbiota

Artículos Relacionados:

Artículo Especial: Simulador gastrointestinal dinámico (simgi®): una herramienta potencialmente útil en nutrición clínica

Trabajo Original: Beneficios de los polifenoles contenidos en la cerveza sobre la microbiota intestinal

Revisión: Situación actual de la obesidad infantil en México

Carta Editor: Hipótesis alternativas sobre los beneficios de los fermentados sobre la microbiota intestinal

Artículo Especial: Los primeros 1000 días: una oportunidad para reducir la carga de las enfermedades no transmisibles

Trabajo Original: Modulación a través del estilo de vida de la microbiota intestinal

Revisión: Immunonutritional contribution of gut microbiota to fatty liver disease

Trabajo Original: Microbiota intestinal y fibra de cereales: evidencia y recomendaciones prácticas

Trabajo Original: Microbiota benefits after inulin and partially hydrolized guar gum supplementation – a randomized clinical trial in constipated women

Revisión: The influence of endotoxemia on the molecular mechanisms of insulin resistance

Revisión: Gut microbiota and the development of obesity

Trabajo Original: Influence of early environmental factors on lymphocyte subsets and gut microbiota in infants at risk of celiac disease; the PROFICEL study

Artículo Especial: Progreso en el conocimiento de la microbiota intestinal humana

Artículo Especial: Aplicaciones clínicas del empleo de probióticos en pediatría

Revisión: Intestinal microbiota; relevance to obesity and modulation by prebiotics and probiotics

Trabajo Original: La obesidad infantil como consecuencia de la interacción entre firmicutes y el consumo de alimentos con alto contenido energético

Revisión: Alimentación funcional para aliviar desórdenes gastrointestinales asociados a trastornos del espectro autista: Una revisión sistemática

Trabajo Original: Effect of voluntary wheel running on gut microbiota composition in developing mice

Revisión: Variación en el aporte de los biocomponentes inmunoglobulina A y lactoferrina en la leche humana después de la pasteurización Holder

Revisión: Nutrición e inmunidad en las primeras etapas de la vida

Revisión: Anorexia nerviosa, microbiota y cerebro

Artículos más populares

Revisión: Ayuno intermitente: efectos en diversos escenarios clínicos

Trabajo Original: Body mass index and risk of inflammatory breast disease: a Mendelian randomization study

Revisión: Relación entre la dieta, aspectos nutricionales y la calidad del sueño en población pediátrica