Nutrición Hospitalaria 03335 / http://dx.doi.org/10.20960/nh.03335

Trabajo Original

In vivo assessment and characterization of lactic acid bacteria with probiotic profile isolated from human milk powder

Ariana Rodríguez Arreola, Josué R. Solís Pacheco, Monique Lacroix, Edgar Balcazar López, Rosa Elena Navarro Hernández, Flavio Sandoval-Garcia, José Alfonso Gutiérrez Padilla, Elisa García Morales, Blanca Rosa Aguilar Uscanga

Prepublicado: 2020-12-15

Publicado: 2021-02-16

VOLUMEN 38, NÚM. 1, enero-febrero (2021), PAG. 152-160

Logo Descargas Número de descargas: 8151 Logo Visitas Número de visitas: 5084 Citas Citas: 0

Compártelo:

Introduction: breast milk (MH) contains nutrients and bioactive compounds for child development, including probiotic bacteria, which contribute to intestinal maturation. This benefit accompanies the individual until adulthood. There are new methods such as spray drying that give this compound a good conservation without loss of microbiota. Objective: the aim of this study was to analyze the viability of lactic acid bacteria isolated from human milk with probiotic potential after the spray drying process, as well as to evaluate the possible adhesion in the colon of mice of the Balb/C strain after feeding them powdered human milk and a commercial formula milk. Method: we isolated and identified the presence of lactic acid bacteria with possible probiotic potential in powdered human milk using the MALDI-TOF MS technique. Powdered human milk and a commercial formula milk were fed to mice of the Bald/C strain for 14 weeks. Glucose level and weight were measured in the mice. The feces were collected to verify the presence of lactic bacteria. The mice were sacrificed and their intestines were weighed, isolating the lactic acid bacteria both from the intestines and from the feces. The strains isolated from mice fed human milk were evaluated for their probiotic potential, analyzing their ability to inhibit pathogens, resistance to pH, temperature, adhesion, and hydrophobicity. Results: the presence of Lactobacillus fermentum LH01, Lactobacillus rhamnosus LH02, Lactobacullis reuteri LH03, and Lactobacillus plantarum LH05 in powdered human milk was identified. All strains showed a possible probiotic profile due to the ability of bacteria to resist low pH, bile salts, and exposure to gastric enzymes, as well as their hydrophobicity and self-aggregation capacity, and their failure to show hemagglutination or hemolysis activity in a culture medium rich in erythrocytes. We observed that the consumption of powdered human milk prevented weight gain and constipation in mice. Conclusions: after spray drying, strains with possible probiotic potential may be preserved in human milk. The consumption of powdered human milk with probiotic bacteria prevents constipation and weight gain in mice, when compared to those fed a commercial formula milk.

Palabras Clave: Human milk. Probiotic. Spray drying. Lactic acid bacteria. Probiotic activity.

Descargar PDF (ENGLISH)

Bibliografía
Artículos Relacionados

Collins SM, Bercik P. The relationship between intestinal microbiota and the central nervous system in normal gastrointestinal function and disease. Gastroenterology 2009;136:2003-14.
DOI: 10.1053/j.gastro.2009.01.075

Davis JCC, Lewis ZT, Krishnan S, Berstein RM, Moore SE, Prentice AM, et al. Growth and morbidity of Gambian infants are influenced by maternal milk oligosaccharides and infant gut microbiota. Scientific Reports 2017;40466(7):1-16.
DOI: 10.1038/srep40466

Jones SE, Versalovic J. Probiotic Lactobacillus reuteri biofilms produce antimicrobial and anti-inflammatory factors. BMC Microbiol 2009;9:1-9.

Newburg DS. Innate immunity and human milk. Journal of Nutrition 2005;135(5):1308-12.
DOI: 10.1093/jn/135.5.1308

Maldonado J, Canabate F, Sempere L, Vela F, Sánchez AR, Narbona E, et al. Human milk probiotic Lactobacillus fermentum CECT5716 reduces the incidence of gastrointestinal and upper respiratory tract infections in infants. J Pediatr Gastroenterol Nutr 2012;1:55-61.
DOI: 10.1097/MPG.0b013e3182333f18

Fernández L, Langaa S, Martína V, Maldonado A, Jiménez E, Martín R, et al. The human milk microbiota: Origin and potential roles in health and disease. Pharmacol Res 2013;69:1-10.
DOI: 10.1016/j.phrs.2012.09.001

Viazis S, Farkas BE, Allen JC. Effects of high-pressure processing on immunoglobulin A and lysozyme activity in human milk. J Hum Lact 2007;23:253-61.
DOI: 10.1177/0890334407303945

Permanyer M, Castellote C, Ramírez SC, Audí C, Pérez CFJ, Castell M, et al. Maintenance of breast milk immunoglobulin an after high-pressure processing. J Dairy Sci 2010;93:877-83.
DOI: 10.3168/jds.2009-2643

Contador R, Delgado AJ, Delgado FJ, Cava R, Ramírez R. Effect of thermal pasteurization or high pressure processing on immunoglobulin and leukocyte contents of human milk. Int Dairy J 2013;32:1-5.
DOI: 10.1016/j.idairyj.2013.03.006

Desmond C, Ross RPE, O'Callaghan G, Fitzgerald C. Improved survival of Lactobacillus paracasei NFBC 338 in spray‐dried powders containing gum acacia. J Appl Microbiol 2002;93:1003-11.
DOI: 10.1046/j.1365-2672.2002.01782.x

Ratti C. Hot air and freeze-draying of high value foods: a review. J Food Eng 2001;49:311-9.
DOI: 10.1016/S0260-8774(00)00228-4

Castro AJ, Aguilar UBR, Calon F, St-Amour I, Solís PJR, Saucier L, Ratti C. Spray and Freeze Drying of Human Milk on the Retention of Immunoglobulins (IgA, IgG, IgM). Dry Technol Int J 2016;15:1801-9.
DOI: 10.1080/07373937.2016.1141781

American Veterinary Medical Association (AVMA). Report of the AVMA Panel on Euthanasia 2000. JAVMA 2001;5:669-96.
DOI: 10.2460/javma.2001.218.669

Serrano NJC, Solís PJR, Gutiérrez PJA, Cobián GA, Cavazos GA, González RO, et al. Isolation and Identification of Lactic Acid Bacteria from Human Milk with Potential Probiotic Role. J Food Nutr Res 2016;3:170-7.

Maldonado N, Robledo C, Robledo J. La espectrometría de masas MALDI-TOF en el laboratorio de microbiología clínica. Rev Infectio 2017;35-45.
DOI: 10.22354/in.v0i0.703

Aguilar UBR, Solís PJR, Plascencia L, García HS, Aguilar MG, Lacroix M. Effect of culture medium on antimicrobial bacteriocin production by Lactobacillus rhamnosus HN001 and Lactobacillus reuteri ATCC 53608. J Microbiol Biotechnol Food Sci 2013;6:2462-8.

Pieniz S, Andreazza R, Anghinoni T, Camargo F, Brandelli A. Probiotic potential, antimicrobial and antioxidant activities of Enterococcus durans strain LAB18s. Food Control 2014;37:251-6.
DOI: 10.1016/j.foodcont.2013.09.055

Botes M, Loos B, van Reenen CA, Dicks LMT. Adhesion of the probiotic strains Enterococcus mundtii ST4SA and Lactobacillus plantarum 423 to Caco-2 cells under conditions simulating the intestinal tract, and in the presence of antibiotics and anti-inflammatory medicaments Marelize. Arch Microbiol 2008;190:573-84.
DOI: 10.1007/s00203-008-0408-0

Del Re B, Sgorbati B, Miglioli M, Palenzona D. Adhesion, autoaggregation and hydrophobicity of 13 strains of Bifidobacterium longum. Lett Appl Microbiol 2000;31:438-42.
DOI: 10.1046/j.1365-2672.2000.00845.x

Kos B, Suskovic SJ, Vukovic M, Simpraga JF, Matosic S. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J Appl Microbiol 2003;94:981-7.
DOI: 10.1046/j.1365-2672.2003.01915.x

Devaki H.R, Kandi RV. Comparison of Hemagglutination and Hemolytic Activity of Various Bacterial Clinical Isolates Against Different Human Blood Groups. Cureus 2016;8(2):489.

Gardiner E, O’sullivan J, Kelly MA, Auty GF, Collins JK, Ross RP. Comparative Survival Rates of Human-Derived Probiotic Lactobacillus paracasei NFBC 338 and L. salivarius UCC118d strains during Heat Treatment and Spray Drying. Appl Environ Microbiol 2000;2605-12.
DOI: 10.1128/AEM.66.6.2605-2612.2000

Rout GK, Jayanta KP, Sushanto G, Yooheon P, Han-Seung S, Gitishree D. Benefaction of probiotics for human health: A review. Journal of food and drug analysis 2018;26:927-39.
DOI: 10.1016/j.jfda.2018.01.002

Murray PR, Rosenthal KS, Pfaller MA. Staphylococcus y cocos grampositivos relacionados. Microbiología Médica, 6ª Ed.; 2009. p. 209-23.

Castillo RF, Hernández CF, Gallegos MG, Flores OA, Rodríguez HR, Aguilar C. Efectividad in vitro de Bacillus y polifenoles de plantas nativas de México sobre Rhizoctonia solani. Rev Mex Cienc Agr 2015;549-62.

Zou J, Jiang H, Cheng H, Fang J, Huang G. Strategies for screening, purification and characterization of bacteriocins. Int J Biol Macromol 2018;117:781-9.
DOI: 10.1016/j.ijbiomac.2018.05.233

Collado MC, Meriluoto J, Salminen S. Adhesion and aggregation properties of probiotic and pathogen strains. Eur Food Res Tech 2008;226:1065-3.
DOI: 10.1007/s00217-007-0632-x

Kos B, Suskovic SJ, Vukovic M, Simpraga JF, Matosic S. Adhesion and aggregation ability of probiotic strain Lactobacillus acidophilus M92. J Appl Microbiol 2003;94(6):981-7.
DOI: 10.1046/j.1365-2672.2003.01915.x

Peighambardoust SH, Golshan TA, Hesari J. Application of spray drying for preservation of lactic acid starter cultures: a review. Trends Food Sci Tech 2011;22:215-24.
DOI: 10.1016/j.tifs.2011.01.009

Paéz R, Lavari L, Audero G, Cuatrin A, Zaritzky N, Reinheimer J, et al. Study of the effects of spray-drying on the functionality of probiotic lactobacilli. J Dairy Tech 2013; 2:155-61.
DOI: 10.1111/1471-0307.12038

Bongers MEJ, Lorijn F, Reitsma JB, Groeneweg M, Taminiau JAJ, Benninga MA. The clinical effect of a new infant formula in term infants with constipation: a double-blind, randomized cross-over trial. Nutr J 2007;8:1-7.
DOI: 10.1186/1475-2891-6-8

Baker P, Smith J, Salmon L, Friel S, Kent G, Lellamo A, et al. Global trends and patterns of commercial milk-based formula sales: ¿is an unprecedented infant and young child feeding transition underway? Public Health Nutr 2016;1-11.
DOI: 10.1017/S1368980016001117

Boehm G, Lidestri M, Casetta P, Jelinek J, Negretti F, Stahl B, et al. Supplementation of a bovine milk formula with an oligosaccharide mixture increases counts of faecal bifidobacteria in preterm infants. Arch Dis Child Fetal Neonatal 2002;86:F178-81.
DOI: 10.1136/fn.86.3.F178

Braegger C, Chmielewska A, Decsi T, Kolacek S, Mihatsch W, Moreno M, et al. Supplementation of Infant Formula with Probiotics and/or Prebiotics: A Systematic Review and Comment by the ESPGHAN. Committee on Nutrition ESPGHAN. JPGN 2011;2:238-50.

Artículos más populares

Revisión: Bebidas de soja y salud femenina. Revisión de la evidencia y opinión de expertos

Las bebidas vegetales de soja constituyen una alte...

Publicado: 2022-07-25

Resumen | PDF

Trabajo Original: Experience with the management of patients requiring home enteral nutrition in routine clinical practice in Spain: the ECONES study

Introduction: enteral nutrition is an effective me...

Publicado: 2023-02-09

Resumen | PDF

Revisión: La dieta como fuente de sulfuro de hidrógeno y sus efectos en la salud y la enfermedad

Conocido originalmente por sus efectos deletéreos...

Publicado: 2022-09-28

Resumen | PDF

Licencia creative commons: Open Access bajo la licencia Creative Commons 4.0 CC BY-NC-SA
https://creativecommons.org/licenses/by-nc-sa/4.0/legalcode

Editado en Madrid por:

Diseño y Desarrollo web Im3diA comunicación

Trabajo Original

In vivo assessment and characterization of lactic acid bacteria with probiotic profile isolated from human milk powder

Artículos Relacionados:

Artículo Especial: Aspectos dietoterapéuticos en situaciones especiales del paciente oncológico. prebióticos y probióticos, ¿tienen cabida en la terapia nutricional del paciente oncológico?

Revisión: Beneficios inmunológicos de la leche humana para la madre y el niño. revisión sistemática

Trabajo Original: Impacto de la pasteurización/lioﬁlización en el contenido disponible de inmunoglobulinas en leche humana madura. estudio de aplicación en bancos de leche humana en hospitales

Trabajo Original: Beneficios a corto, medio y largo plazo de la ingesta de leche humana en recién nacidos de muy bajo peso

Trabajo Original: Papel de los prebióticos y los probióticos en la funcionalidad de la microbiota del paciente con nutrición enteral

Trabajo Original: Concentration of ghrelin and leptin in serum and human milk in nursing mothers according to the type of feeding

Trabajo Original: Influencia de la dieta de mujeres mexicanas sobre la calidad nutricional y la presencia de microorganismos benéficos en la leche humana

Trabajo Original: Real-world study in infants fed with an infant formula with two human milk oligosaccharides

Trabajo Original: Nutrición y microbiota en población pediátrica. Implicaciones sanitarias

Trabajo Original: Simbiosis en el tracto gastrointestinal humano

Trabajo Original: Impacto de la pandemia por SARS-CoV-2 en un banco de leche humana

Revisión: Efecto de la suplementación con omega-3 durante la gestación y la lactancia sobre la composición de ácidos grasos de la leche materna en los primeros meses de vida: una revisión narrativa

Artículo Especial: Controversia actual sobre el papel crítico de los ácidos grasos poliinsaturados de cadena larga, araquidónico (ARA) y docosahexaenoico (DHA), en el lactante

Revisión: Revising concepts of artificial nutrition in contemporary surgery: from energy and nitrogen to immuno-metabolic support

Trabajo Original: A mixture of Lactobacillus plantarum CECT 7315 and CECT 7316 enhances systemic immunity in elderly subjects. A dose-response, double-blind, placebo-controlled, randomized pilot trial

Trabajo Original: El consumo del probiótico Lactobacillus plantarum CECT 7315/7316 mejora el estado de salud general en personas de edad avanzada

Trabajo Original: Estabilidad de la capacidad antioxidante y pH en leche humana refrigerada durante 72 horas: estudio longitudinal

Revisión: The effect of probiotics on the serum lipid levels in non-obese healthy adults with hyperlipidemia: A systematic review and meta-analysis of randomized controlled trials

Trabajo Original: Is there a relationship between oral hygiene and nutritional status in peritoneal dialysis patients?

Revisión: Effect of the use of probiotics in the treatment of children with atopic dermatitis; a literature review

Artículo Especial: Third Jesús Culebras Lecture - Molecular Biology and Clinical Nutrition; where do we stand and where do we go?

Artículo Especial: Probióticos en las enfermedades hepáticas

Artículo Especial: Aplicaciones clínicas del empleo de probióticos en pediatría

Revisión: Intestinal microbiota; relevance to obesity and modulation by prebiotics and probiotics

Revisión: Lactobacillus Paracasei subsp. Paracasei F19; a farmacogenomic and clinical update

Trabajo Original: Probióticos y enterocolitis necrotizante del prematuro; to NEC or not to NEC?, ésta es la pregunta

Trabajo Original: Effects of human milk on blood and bone marrow cells in a malnourished mice model; comparative study with cow milk

Trabajo Original: Effect of probiotics on human blood urea levels in patients with chronic renal failure

Trabajo Original: Nueva guía de práctica clínica sobre nutrición enteral del recién nacido de muy bajo peso al nacimiento; segunda parte

Trabajo Original: The effect of Saccharomyces boulardii in patients eligible for liver transplantation

Trabajo Original: Are fat acids of human milk impacted by pasteurization and freezing?

Revisión: Alimentación funcional para aliviar desórdenes gastrointestinales asociados a trastornos del espectro autista: Una revisión sistemática

Trabajo Original: Determination of cholesterol in human milk: an alternative to chromatographic methods

Revisión: Probiotics in the treatment of infantile colic: a meta-analysis of randomized controlled trials

Trabajo Original: Influencia del perfil de las donantes en la bacteriología pre- y pospasteurización de la leche humana donada

Revisión: Bifidobacterium longum subsp. infantis CECT 7210 (B. infantis IM-1®) muestra actividad frente a patógenos intestinales

Revisión: Treating asthma patients with probiotics: a systematic review and meta-analysis

Revisión: Variación en el aporte de los biocomponentes inmunoglobulina A y lactoferrina en la leche humana después de la pasteurización Holder

Trabajo Original: Production of snack bar enriched with paraprobiotics grown in banana peel medium, nutritional, sensory and quality parameters

Artículos más populares

Revisión: Bebidas de soja y salud femenina. Revisión de la evidencia y opinión de expertos

Trabajo Original: Experience with the management of patients requiring home enteral nutrition in routine clinical practice in Spain: the ECONES study

Revisión: La dieta como fuente de sulfuro de hidrógeno y sus efectos en la salud y la enfermedad