Número de descargas: 2999 Número de visitas: 5732 Citas: 0
Compártelo:
Introduction: Polychlorinated biphenyls (PCBs), used as pesticides in agriculture, can lead to irreversible injuries in living organisms, particularly in liver. Oxidative stress has been implicated in the liver pathogenesis induced by different molecules, including PCBs. It has been demonstrated that quercetin, an antioxidant flavonoid found in the diet, exhibits a potent antioxidant effect in different liver pathologies. Objective: To evaluate oxidative stress caused by PCBs in liver and the antioxidant activity of quercetin. Methodology: We used male Wistar rats (n = 36), divided in 4 groups: control, quercetin (50 mg/kg/day), PCBs (0.4 ml/kg/day), and rats treated with both PCBs and quercetin. On day 25 blood was collected to assess liver integrity (enzymes AST, ALT and ALP), and liver samples to measure oxidative stress (TBARS), activity of antioxidant enzymes (SOD, CAT, GPx) and DNA damage (micronucleus assay), and histological damage. Results: TBARS concentration and SOD activity were significantly higher in PCBs animals as compared to the PCB group receiving quercetin. CAT and GPx decreased in PCBs and increased when quercetin was added. The histological analysis showed damage to hepatocytes in PCBs, but quercetin was able to afford protection against such damage. The micronucleus test showed there was an increase in the production of microclenucleus compared to control, and quercetin was able to reduce this effect. Conclusion: Contamination with PCBs led to increased lipid peroxidation and DNA damage, and the use of antioxidant quercetin was effective in reducing PCBs-induced liver injury.
Palabras Clave: Hepatic toxicity. Pesticides. Antioxidants.
Elizângela Gonçalves Schemitt , Josieli Raskopf Colares , Renata Minuzzo Hartmann , María Isabel Morgan-martins , Cláudio Augusto Marroni , M. Jesús Tuñón , Norma Possa Marroni
Karolina Álvarez-altamirano , Alma Nubia Mendoza-hernández , Carolina Carcoba-tenorio , José Antonio García-garcía , Vanessa Fuchs-Tarlovsky
Patricia Carolina Castillo-castañeda , Ramón Gaxiola-robles , Lía Celina Méndez-rodríguez , Vanessa Labrada-martagón , Tania Zenteno-savín
Rosa M. Ortega Anta , Ana Isabel Jiménez-Ortega , José Miguel Perea Sánchez , Esther Cuadrado Soto , Ana M. López Sobaler
Elena Rodríguez-Rodríguez , Paula Rodríguez-rodríguez , Liliana Guadalupe González-Rodríguez , Ana María López Sobaler
Ana Fernández-araque , Andrea Giaquinta Aranda , Abel Rojo Aragüés
Ilya A. Volchegorskii , Elena V. Tur
Aránzazu Aparicio , María Dolores Salas-González , Esther Cuadrado-Soto , Rosa M. Ortega , Ana M. López-Sobaler
Ulil Us Medina , Maria del Carmen Millán Linares , Victor Arana Argáez , Maira Rubi Segura-Campos
Tonancy Nicolás-Méndez , Alda Rocío Ortiz-Muñiz , Víctor Manuel Mendoza-Núñez , María del Carmen García-Rodríguez
Priscila Stefani Vallejos Vildoso , Jorge da Silva Pinho Jr , Ana Paula Azevedo de Mattos , Gabrielle de Souza Rocha , Sergio Girão Barroso , Grazielle Vilas Boas Huguenin , Andréa Cardoso de Matos
Vivian Alves Pereira Silva , Gilson Teles Boaventura , Renato de Souza Abboud , José Antônio Silva Ribas , Mauricio Alves Chagas
Javier Alves Vas , Gema Barrientos , Víctor Toro Román , María Concepción Robles Gil , Marcos Maynar Mariño , Diego Muñoz Marín
Elena Rodríguez-Rodríguez , Aránzazu Aparicio , Laura M. Bermejo , Rosa M. Ortega , Ana M. López-Sobaler
Tuğba Küçükkasap Cömert , Özlem Muşlu , Duygu Ağagündüz
Siyu Liu , Junhui Chen , Yiwen Wang , Yangyang Xu
Antecedentes y objetivo: la endometriosis es un tr...