Número de descargas: 4822 Número de visitas: 1777 Citas: 0
Compártelo:
Purine nucleoside phosphorylase (PNP) is an ubiquitous enzyme which plays an important role in arsenic (As) detoxification. As is a toxic metalloid present in air, soil and water; is abundant in the environment and is readily transferred along the trophic chain, being found even in human breast milk. Milk is the main nutrient source for the growth and development of neonates. Information on breast milk synthesis and its potential defense mechanism against As toxicity is scarce. In this study, PNP and antioxidant enzymes activities, as well as glutathione (GSH) and total arsenic (TAs) concentrations, were quantified in breast milk samples. PNP, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST), glutathione peroxidase (GPx), glutathione reductase (GR) activities and GSH concentration were determined spectrophotometrically; TAs concentration ([TAs]) was measured by atomic absorption spectrometry. Data suggest an increase in PNP activity (median = 0.034 U mg protein-1) in the presence of TAs (median = 1.16 g L-1). To explain the possible association of PNP activity in breast milk with the activity of the antioxidant enzymes as well as with GSH and TAs concentrations, generalized linear models were built. In the adjusted model, GPx and GR activities showed a statistically significant (p<0.01) association with PNP activity. These results may suggest that PNP activity increases in the presence of TAs as part of the detoxification mechanism in breast milk.
Palabras Clave: Arsenic. Breast milk. Generalized linear model. Oxidative stress. Purine nucleoside phosphorylase.
Ernesto Cortés-Castell , Carmen Veciana-galindo , Luis Torró-montell , Antonio Palazón-Bru , Elia Sirvent-Segura , Vicente Gil-guillén , Mercedes Rizo-baeza
Elizângela Gonçalves Schemitt , Josieli Raskopf Colares , Renata Minuzzo Hartmann , María Isabel Morgan-martins , Cláudio Augusto Marroni , M. Jesús Tuñón , Norma Possa Marroni
María Teresa Nestares , Macarena Salinas , Carlos De Teresa , Javier Díaz Castro , Jorge Moreno-Fernández , Magdalena López-frías
Sergio Cuellar-rufino , Mónica Navarro-meza , Pablo García-solís , Irene Xochihua-rosas , Omar Arroyo-helguera
Amelia Marti , Raquel Echeverría , Lydia Morell-azanza , Ana Ojeda-rodríguez
Rebeca Monroy Torres , Antonio Espinosa Pérez , Xochitl Ramírez Gómez , Leticia Carrizales Yáñez , Benigno Linares Segovia , Jesús Mejía Saavedra
Dayana Joyce Marques Queiroz , Alexandre Sérgio Silva , Alcides da Silva Diniz , Alice Teles de Carvalho , Eduarda Pontes dos Santos Araújo , Juliana Padilha Ramos Neves , Lavoisiana Lacerda de Lucena , Lydiane Tavares Toscano , Maria da Conceição Rodrigues Gonçalves
Natália Yumi Noronha , Marcela Augusta De Souza Pinhel , Carolina Ferreira Nicoletti , Driele Cristina Gomes Quinhoneiro , Vitor Caressato Pinhanelli , Bruno Affonso Parenti Oliveira , Cristiana Cortes-Oliveira , Heitor Bernardes Pereira Delfino , Leticia Santana Wolf , Fabiani Gai Frantz , Júlio Sergio Marchini , Carla Barbosa Nonino
Jesús Siquier Coll , Diego Muñoz Marín , Francisco Javier Grijota Pérez , Ignacio Bartolomé Sánchez , María Concepción Robles Gil , Julio Montero Arroyo , Marcos Maynar Mariño
Casilda Olveira , Eva García-Escobar , Esperanza Doña , Francisco J. Palenque , Nuria Porras , Antonio Dorado , Ana María Godoy , Elezahara Rubio-Martín , Francisco-Javier Bermúdez-Silva , Silvana Y. Romero-Zerbo , Gemma Rojo Martínez , Rocío Martín-Valero , José Abuín Fernández , Gabriel Olveira Fuster
Adriana Rodríguez-San Nicolás , Martha A. SÁnchez-RodrÍguez , Mariano Zacarías-Flores , Elsa Correa-Muñoz , Víctor Manuel Mendoza-Núñez
Blanca Rosa Aguilar Uscanga , Adriana Cavazos Garduño , Josué R. Solís Pacheco , Flavio Sandoval-Garcia , Rosa Elena Navarro Hernández , Julio César Serrano Niño
Maria Jesus Lisbona-González , Candela Reyes Botella , Esther Muñoz Soto , Marta Vallecillo-Rivas , Jorge Moreno Fernández , Javier Díaz Castro
Priscila Stefani Vallejos Vildoso , Jorge da Silva Pinho Jr , Ana Paula Azevedo de Mattos , Gabrielle de Souza Rocha , Sergio Girão Barroso , Grazielle Vilas Boas Huguenin , Andréa Cardoso de Matos
Claudia Bento , Andrea Matos , Adryana Cordeiro , Andréa Ramalho
Rocío Angélica Salinas Osornio , María Victorina Aguilar-Vilas , Antonio Becerra-Fernández , Laura González López , Blanca Miriam Torres-Mendoza
Ximena León-Lara , Ariana Vargas-Castillo , Azalia Avila-Nava , Martha Guevara-Cruz , Aurora E Serralde Zúñiga , Isabel Medina-Vera
Lavoisiana Lacerda de Lucena , Alexandre Sérgio Silva , Rayner Anderson Ferreira do Nascimento , Darlene Camati Persuhn , Juliana Padilha Ramos Neves , Maria José Carvalho Costa , Dayana Joyce Marques Queiroz , Rafaela Lira Formiga Covalcanti de Lima , Raquel Patrícia Ataíde de Lima , Maria Paula de Paiva , Naila Francis Paulo de Oliveira , Maria da Conceição Rodrigues Gonçalves
Samara Bomfim Gomes Campos , Amylly Sanuelly da Paz Martins , Anne Karolyne dos Santos , Marília Oliveira Fonseca Goulart , Fabiana Andréa Moura
Aina Tomás Luiz , Gala Martín Pozuelo , Inmaculada González Navarro , Laura Elvira Torales , Helena Ponce , Rocío González Barrio , Javier García Alonso , María Jesús Periago
Rosa M. Ortega , Ana Isabel Jiménez-Ortega , Rosa María Martínez García , Ana María Lorenzo-Mora , María del Carmen Lozano-Estevan
Blanca M. G. Torres-Mendoza , Genaro Gabriel Ortiz , Lorenzo Sánchez-Romero , Daniela L. C. Delgado-Lara , María T. García Martínez , Mario-Alberto Mireles-Ramírez , José Antonio Cruz Serrano , Fermín Paul Pacheco Moisés
Dayana Joyce Marques Queiroz , Alexandre Sérgio Silva , Celso Costa da Silva Júnior , Darlene Camati Persuhn , Alcides da Silva Diniz , Rafaela Lira Formiga Cavalcanti de Lima , Maria Paula de Paiva , Constantino Giovanni Braga Cartaxo , Patricia Gomes de Matos Bezerra , Mateus Duarte Ribeiro , Maria da Conceição Rodrigues Gonçalves
Maria Goretti Reyes López , Adriana Cavazos Garduño , Nancy Elizabeth Franco Rodríguez , Jorge Manuel Silva Jara , Julio César Serrano Niño
José Antonio Palma-Jacinto , Isela Santiago-Roque , María del Rocío Coutiño-Rodríguez , Omar Elind Arroyo-Helguera